Detailed information of TA system TA224050

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96968..97237
• Replicon: plasmid pRIVM_C039629_1
• Accession: NZ_CP086617
• Organism: Escherichia coli strain RIVM_C039629

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LN353_RS25495
• Protein ID: WP_001372321.1
• Coordinates: 97112..97237 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	96968..97033 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN353_RS25455	92043..92291	+	249	WP_071606928.1	hypothetical protein	-
LN353_RS25460	92761..93288	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
LN353_RS25465	93344..93577	+	234	WP_000006018.1	DUF905 domain-containing protein	-
LN353_RS25470	93636..95594	+	1959	WP_032152921.1	ParB/RepB/Spo0J family partition protein	-
LN353_RS25475	95649..96083	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
LN353_RS25480	96080..96842	+	763	Protein_122	plasmid SOS inhibition protein A	-
LN353_RS25485	96811..96999	-	189	WP_001299721.1	hypothetical protein	-
-	96811..97035	+	225	NuclAT_0	-	-
-	96811..97035	+	225	NuclAT_0	-	-
-	96811..97035	+	225	NuclAT_0	-	-
-	96811..97035	+	225	NuclAT_0	-	-
-	96968..97033	-	66	-	-	Antitoxin
LN353_RS25490	97021..97170	+	150	Protein_124	plasmid maintenance protein Mok	-
LN353_RS25495	97112..97237	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LN353_RS25500	97457..97687	+	231	WP_071587244.1	hypothetical protein	-
LN353_RS25505	97685..97858	-	174	Protein_127	hypothetical protein	-
LN353_RS25510	98156..98443	+	288	WP_000107537.1	hypothetical protein	-
LN353_RS25515	98564..99385	+	822	WP_001234469.1	DUF932 domain-containing protein	-
LN353_RS25520	99682..100329	-	648	WP_124760006.1	transglycosylase SLT domain-containing protein	-
LN353_RS25525	100615..100998	+	384	WP_001151564.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LN353_RS25530	101192..101878	+	687	WP_000332487.1	PAS domain-containing protein	-
LN353_RS25535	101972..102199	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27	-	1..138402	138402

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T224050 WP_001372321.1 NZ_CP086617:97112-97237 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T224050 NZ_CP119577:3541858-3542076 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT224050 NZ_CP086617:c97033-96968 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin