Detailed information of TA system TA224047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4963746..4964348
• Replicon: chromosome
• Accession: NZ_CP086616
• Organism: Escherichia coli strain RIVM_C039629

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y9BEM6
• Locus tag: LN353_RS23910
• Protein ID: WP_059332311.1
• Coordinates: 4964037..4964348 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN353_RS23905
• Protein ID: WP_000356395.1
• Coordinates: 4963746..4964036 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN353_RS23870 (4959370)	4959370..4960272	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LN353_RS23875 (4960269)	4960269..4960904	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LN353_RS23880 (4960901)	4960901..4961830	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LN353_RS23885 (4962012)	4962012..4962254	-	243	WP_001309881.1	CopG family transcriptional regulator	-
LN353_RS23890 (4962473)	4962473..4962691	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LN353_RS23895 (4963110)	4963110..4963388	-	279	WP_001315112.1	hypothetical protein	-
LN353_RS23900 (4963440)	4963440..4963661	-	222	WP_001550354.1	hypothetical protein	-
LN353_RS23905 (4963746)	4963746..4964036	-	291	WP_000356395.1	NadS family protein	Antitoxin
LN353_RS23910 (4964037)	4964037..4964348	-	312	WP_059332311.1	hypothetical protein	Toxin
LN353_RS23915 (4964577)	4964577..4965485	+	909	WP_001550353.1	alpha/beta hydrolase	-
LN353_RS23920 (4965653)	4965653..4966567	-	915	WP_109553727.1	transposase	-
LN353_RS23925 (4966580)	4966580..4967467	-	888	Protein_4681	hypothetical protein	-
LN353_RS23930 (4967883)	4967883..4968824	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LN353_RS23935 (4968869)	4968869..4969306	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12212.25 Da
• Isoelectric Point: 10.0241

>T224047 WP_059332311.1 NZ_CP086616:c4964348-4964037 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T224047 NZ_CP119577:c2441024-2440848 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT224047 WP_000356395.1 NZ_CP086616:c4964036-4963746 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT224047 NZ_CP119577:c2440802-2440386 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A4Y9BEM6