Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4079199..4079893 | Replicon | chromosome |
| Accession | NZ_CP086616 | ||
| Organism | Escherichia coli strain RIVM_C039629 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | LN353_RS19735 | Protein ID | WP_001263491.1 |
| Coordinates | 4079199..4079597 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | LN353_RS19740 | Protein ID | WP_000554755.1 |
| Coordinates | 4079600..4079893 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4075028) | 4075028..4075108 | - | 81 | NuclAT_10 | - | - |
| - (4075028) | 4075028..4075108 | - | 81 | NuclAT_10 | - | - |
| - (4075028) | 4075028..4075108 | - | 81 | NuclAT_10 | - | - |
| - (4075028) | 4075028..4075108 | - | 81 | NuclAT_10 | - | - |
| LN353_RS19705 (4074368) | 4074368..4075612 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| LN353_RS19710 (4075704) | 4075704..4076162 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LN353_RS19715 (4076423) | 4076423..4077880 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| LN353_RS19720 (4077937) | 4077937..4078289 | - | 353 | Protein_3868 | peptide chain release factor H | - |
| LN353_RS19725 (4078285) | 4078285..4078491 | - | 207 | Protein_3869 | RtcB family protein | - |
| LN353_RS19730 (4078737) | 4078737..4079189 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| LN353_RS19735 (4079199) | 4079199..4079597 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LN353_RS19740 (4079600) | 4079600..4079893 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LN353_RS19745 (4079945) | 4079945..4081000 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| LN353_RS19750 (4081071) | 4081071..4081856 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| LN353_RS19755 (4081828) | 4081828..4083540 | + | 1713 | Protein_3875 | flagellar biosynthesis protein FlhA | - |
| LN353_RS19760 (4083645) | 4083645..4083923 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| LN353_RS19765 (4083916) | 4083916..4084272 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4069135..4079893 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T224043 WP_001263491.1 NZ_CP086616:c4079597-4079199 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T224043 NZ_CP119577:1860253-1860504 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT224043 WP_000554755.1 NZ_CP086616:c4079893-4079600 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT224043 NZ_CP119577:1860501-1860755 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |