Detailed information of TA system TA224028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1318772..1319499
• Replicon: chromosome
• Accession: NZ_CP086616
• Organism: Escherichia coli strain RIVM_C039629

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: LN353_RS06430
• Protein ID: WP_000547555.1
• Coordinates: 1318772..1319083 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LN353_RS06435
• Protein ID: WP_000126294.1
• Coordinates: 1319080..1319499 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN353_RS06405 (1314707)	1314707..1316416	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
LN353_RS06410 (1316426)	1316426..1316968	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LN353_RS06415 (1316968)	1316968..1317735	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LN353_RS06420 (1317732)	1317732..1318142	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LN353_RS06425 (1318135)	1318135..1318605	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
LN353_RS06430 (1318772)	1318772..1319083	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
LN353_RS06435 (1319080)	1319080..1319499	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LN353_RS06440 (1319578)	1319578..1321002	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
LN353_RS06445 (1321011)	1321011..1322468	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LN353_RS06450 (1322728)	1322728..1323738	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
LN353_RS06455 (1323887)	1323887..1324414	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T224028 WP_000547555.1 NZ_CP086616:1318772-1319083 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224028 NZ_CP119571:c1378537-1378175 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT224028 WP_000126294.1 NZ_CP086616:1319080-1319499 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224028 NZ_CP119571:c1378704-1378534 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: V0YLE2