Detailed information of TA system TA224003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4429407..4430086
• Replicon: chromosome
• Accession: NZ_CP086604
• Organism: Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: LNN29_RS21650
• Protein ID: WP_002431667.1
• Coordinates: 4429407..4429709 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN29_RS21655
• Protein ID: WP_000806440.1
• Coordinates: 4429745..4430086 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN29_RS21625 (4424783)	4424783..4426435	+	1653	WP_104919592.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
LNN29_RS21630 (4426473)	4426473..4426976	-	504	WP_000667001.1	hypothetical protein	-
LNN29_RS21635 (4426973)	4426973..4427629	-	657	WP_181674165.1	hypothetical protein	-
LNN29_RS21640 (4427797)	4427797..4428276	-	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
LNN29_RS21645 (4428480)	4428480..4429274	-	795	WP_046081753.1	TraB/GumN family protein	-
LNN29_RS21650 (4429407)	4429407..4429709	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN29_RS21655 (4429745)	4429745..4430086	+	342	WP_000806440.1	HigA family addiction module antitoxin	Antitoxin
LNN29_RS21660 (4430200)	4430200..4432704	-	2505	WP_000083946.1	copper-exporting P-type ATPase CopA	-
LNN29_RS21665 (4433011)	4433011..4434300	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T224003 WP_002431667.1 NZ_CP086604:4429407-4429709 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T224003 NZ_CP119552:c917940-917737 [Providencia stuartii]
ATGACCAAAACTGATTATCTGATGCGTTTAAGAAAATGCACAACCATCGATACACTTGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTTTCTGAAGACGAGCTGGAACTATTTTACTCAGCCGCAGATCACCGTCTTGCAGAACTCACTATGAATAAAC
TTTATGACAAAATTCCAGCCTCTGTTTGGAAATTTGTTAGATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT224003 WP_000806440.1 NZ_CP086604:4429745-4430086 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT224003 NZ_CP119552:c918387-918019 [Providencia stuartii]
ATGGATGAATATTCACCCAAAAATTATGATATATCAGAGTTAAAATACTTGTGTAACAGTTTGAATAGAGAAGCAATTTC
AAGCTTACAGAAAACAAATACACATTGGATAAATGATTTAAGCTCACCACAAAGTGTACGGCTCAATGAGCTTATTGAAC
ATATAGCCGCCTTTGTATGGCAATATAAGATCAAACATCCAAAAGATAATCTCGTTATATCATTAGTAGAAGAGTATCTT
GACGAGACTTACGATTTATTCGGTAGTCCGGTAATAACGTTAAGTGAAATTATTGACTGGCAGAGTATGAATCAAAATCT
TGTCGCTGTACTTGATGATGATTTAAAATGCCCTGCAAGCAAGACCTAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8