Detailed information of TA system TA224001

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4181885..4182131
• Replicon: chromosome
• Accession: NZ_CP086604
• Organism: Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)

• Gene name: hokX
• Uniprot ID: S1PD89
• Locus tag: LNN29_RS20295
• Protein ID: WP_000956458.1
• Coordinates: 4181979..4182131 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokX
Locus tag	-
Coordinates	4181885..4181937 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN29_RS20280	4177393..4179192	+	1800	WP_104920142.1	NADPH-dependent assimilatory sulfite reductase flavoprotein subunit	-
LNN29_RS20285	4179192..4180904	+	1713	WP_104920141.1	assimilatory sulfite reductase (NADPH) hemoprotein subunit	-
LNN29_RS20290	4180978..4181712	+	735	WP_046083134.1	phosphoadenosine phosphosulfate reductase	-
-	4181885..4181937	-	53	-	-	Antitoxin
LNN29_RS20295	4181979..4182131	+	153	WP_000956458.1	Hok/Gef family protein	Toxin
LNN29_RS20300	4182324..4185024	+	2701	Protein_3957	CRISPR-associated helicase Cas3'	-
LNN29_RS20305	4185490..4187052	+	1563	WP_104920139.1	type I-E CRISPR-associated protein Cse1/CasA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (5-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5558.77 Da
• Isoelectric Point: 7.6757

>T224001 WP_000956458.1 NZ_CP086604:4181979-4182131 [Escherichia fergusonii]
MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVLAYESKK

Nucleotide

• Length: 153 bp

>T224001 NZ_CP119546:4390558-4391202 [Providencia stuartii]
ATGGCGTATATGAACCTAAACCCAGAAAAAGCACTTATCTGGAGAATAACCCATCGTCAGAATATTCCTTGGCTTTTTGC
CAATGGCTTATATGCAGGAAATAGTGAACATTATTCACCTAATTGGGTGACTATTGGCAATACAGACCTGATTAACCGCC
GCAATCAACGTCATGTACCTATTCAGCCGAATGGGGTACTTAATGATTATGTACCTTTCTATTTCACCCCTTTCTCGCCA
ATGATGTATAATATTTATACAGGCCGTGGTGGGGTGCGAAAAGTTCCCAATGATGATATTATCATCTTGGTTTCCAGCAT
GTATAAAATAGCCGCTCTAAAGATACCTTTTGTTTTCACCGATCGTCATGCGTATCCTGTAACATCTCAGTTCTATAACC
AGCTAACAGATCTCGCGGAGATAGATTGGGCTTTACTACAGCAGCGCAATTTTCACCGTGACCCTGATGACCCTGAAAAA
GTTGAAAGGTATCAAGCAGAAGCGTTAATTTATAAACATGTTCCTATGGAAGCCCTATTAGGTGCTGTTTGTTACACAAA
AGAAACCCAGCTAGAACTTCAAGCAAAAGCCAGCCAACTTGAGATTCATTTAGATATCCATTGCATTCCAAACTGGTACT
TTTAA

Antitoxin

Nucleotide

• Length: 53 bp

>AT224001 NZ_CP086604:c4181937-4181885 [Escherichia fergusonii]
TTAGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGT

Structures

Toxin

• AlphaFold DB: A0A4P7TJ83

Antitoxin