Detailed information of TA system TA223996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3778381..3779180
• Replicon: chromosome
• Accession: NZ_CP086604
• Organism: Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: LNN29_RS18310
• Protein ID: WP_000347270.1
• Coordinates: 3778381..3778845 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: LNN29_RS18315
• Protein ID: WP_104919918.1
• Coordinates: 3778845..3779180 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN29_RS18280 (3773382)	3773382..3773816	-	435	WP_032303830.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
LNN29_RS18285 (3773834)	3773834..3774712	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
LNN29_RS18290 (3774702)	3774702..3775481	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LNN29_RS18295 (3775492)	3775492..3775965	-	474	WP_032303829.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LNN29_RS18300 (3775988)	3775988..3777268	-	1281	WP_104917761.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LNN29_RS18305 (3777517)	3777517..3778326	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
LNN29_RS18310 (3778381)	3778381..3778845	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LNN29_RS18315 (3778845)	3778845..3779180	-	336	WP_104919918.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LNN29_RS18320 (3779329)	3779329..3780900	-	1572	WP_104919919.1	galactarate dehydratase	-
LNN29_RS18325 (3781371)	3781371..3782141	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
LNN29_RS18330 (3782166)	3782166..3783056	+	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T223996 WP_000347270.1 NZ_CP086604:c3778845-3778381 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T223996 NZ_CP119540:1536246-1536656 [Providencia stuartii]
GTGGTCCTGTGGAAATCTAATCTTTCCATTTCATGGAAGACACAACTTTTTTCAACCTGTGCACACGGGCTGGTAGGTTT
TATTTTATTGGTAGCACCGTGGGCGCCAGGTAATTCCATGGTCTGGTTACCTTTACTTGCCATTGTGATTGCAAGTTGGG
CTAAAAGCCAAAAAAGTATCAGTAAAATTAAAGGCACCGCAGTGCTCGTGAATGGCAACAAGGTGCAATGGAAAAAAAAC
GAGTGGAATATTATTAAGCAACCGTGGTGTAGTCGTGTTGGTATATTATTGACGCTAAGTGCACTACAAGGTAAGCAACA
AAAAATCCGCTTATGGATAGCAAAGGATGCGTTATCTGAAGAAAGCTGGCGTAATTTAAATCAACTATTACTGCAATATC
CAGATATTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12349.93 Da
• Isoelectric Point: 4.5392

>AT223996 WP_104919918.1 NZ_CP086604:c3779180-3778845 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGRKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT223996 NZ_CP119540:1535999-1536265 [Providencia stuartii]
ATGGATATAGAGAACAAAGCAAGAATTCATTGGGCTTGCCGCCGTGGTATGCGTGAATTGGATATTTCCATCATGCCATT
CTTTGAGTATGAGTATGATAGCCTTTCCGATAAGGACAAACAGACTTTCGTCCGTTTACTTGAGTGCGCTGATCCAGACC
TATTTAACTGGTTAATGAATCACGGGCGTCCAGATGATGAAGAGTTGTTTGGTATGGTTAAATTGATTCAGGAAAGAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90