Detailed information of TA system TA223994

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2979151..2979408
• Replicon: chromosome
• Accession: NZ_CP086604
• Organism: Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)

• Gene name: hokA
• Uniprot ID: F4T5C6
• Locus tag: LNN29_RS14515
• Protein ID: WP_001135731.1
• Coordinates: 2979151..2979303 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokA
Locus tag	-
Coordinates	2979360..2979408 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN29_RS14485	2975017..2975727	-	711	WP_000190514.1	DUF3053 domain-containing protein	-
LNN29_RS14490	2976165..2977535	+	1371	WP_104920428.1	MFS transporter	-
LNN29_RS14495	2977785..2978075	+	291	WP_000455800.1	HTH-type transcriptional regulator	-
LNN29_RS14500	2978356..2978568	+	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
LNN29_RS14505	2978622..2978993	-	372	WP_104920427.1	membrane protein insertion efficiency factor YidD	-
LNN29_RS14510	2979103..2979174	+	72	WP_216665726.1	hypothetical protein	-
LNN29_RS14515	2979151..2979303	-	153	WP_001135731.1	type I toxin-antitoxin system toxin HokA	Toxin
-	2979360..2979408	+	49	-	-	Antitoxin
LNN29_RS14520	2979625..2981694	-	2070	WP_001291786.1	glycine--tRNA ligase subunit beta	-
LNN29_RS14525	2981704..2982615	-	912	WP_001168544.1	glycine--tRNA ligase subunit alpha	-
LNN29_RS14530	2982711..2983010	-	300	WP_024256552.1	YsaB family lipoprotein	-
LNN29_RS14535	2983182..2984177	+	996	WP_002432855.1	acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (5-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 6025.30 Da
• Isoelectric Point: 7.7169

>T223994 WP_001135731.1 NZ_CP086604:c2979303-2979151 [Escherichia fergusonii]
MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQENYELAAFLACKLKE

Nucleotide

• Length: 153 bp

>T223994 NZ_CP119540:713047-713985 [Providencia stuartii]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGACTGCTTCCTGTGGTAGCAACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAAATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATCGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGAAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGTATGTTACTTGGCAGTGAAGGGATTTCGAGAGAGATTTTGGTGGGTTTTTTAACCTA
TACATTAAGTCACCCTCAGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCATCTGAATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCCATGATGTTAATTGAACTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGACTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAGTTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACATCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin

Nucleotide

• Length: 49 bp

>AT223994 NZ_CP086604:2979360-2979408 [Escherichia fergusonii]
GCATAGAGGGGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Structures

Toxin

• AlphaFold DB: A0A061YDD9

Antitoxin