Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1376047..1376549 Replicon chromosome
Accession NZ_CP086604
Organism Escherichia fergusonii strain EF20JDJ4045

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag LNN29_RS06740 Protein ID WP_000767829.1
Coordinates 1376047..1376301 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LNN29_RS06745 Protein ID WP_001259255.1
Coordinates 1376298..1376549 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN29_RS06710 (1371064) 1371064..1371291 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
LNN29_RS06715 (1371305) 1371305..1372363 - 1059 WP_104920325.1 thiosulfate utilization transporter TsuA/YeeE -
LNN29_RS06720 (1372542) 1372542..1373900 - 1359 WP_000019201.1 putrescine/proton symporter PlaP -
LNN29_RS06725 (1373890) 1373890..1373952 - 63 WP_010723108.1 membrane protein YoeI -
LNN29_RS06730 (1374166) 1374166..1375095 - 930 WP_104920324.1 LysR substrate-binding domain-containing protein -
LNN29_RS06735 (1375140) 1375140..1375964 - 825 WP_000754726.1 SDR family oxidoreductase -
LNN29_RS06740 (1376047) 1376047..1376301 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LNN29_RS06745 (1376298) 1376298..1376549 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LNN29_RS06750 (1376833) 1376833..1376883 + 51 WP_001364200.1 his operon leader peptide -
LNN29_RS06755 (1377029) 1377029..1377928 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
LNN29_RS06760 (1377934) 1377934..1379238 + 1305 WP_015953522.1 histidinol dehydrogenase -
LNN29_RS06765 (1379235) 1379235..1380305 + 1071 WP_000108922.1 histidinol-phosphate transaminase -
LNN29_RS06770 (1380305) 1380305..1381372 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T223986 WP_000767829.1 NZ_CP086604:c1376301-1376047 [Escherichia fergusonii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T223986 NZ_CP119531:c3434794-3434691 [Raoultella electrica]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT223986 WP_001259255.1 NZ_CP086604:c1376549-1376298 [Escherichia fergusonii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT223986 NZ_CP119531:3434686-3434852 [Raoultella electrica]
CAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGCGTCAGGTTTTCCAGTCCGCAACAAAAGTGGT
CGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References