Detailed information of TA system TA022397

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 11129..11546
• Replicon: plasmid pETEC_74
• Accession: NC_009790
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECE24377A_RS01235
• Protein ID: WP_001312861.1
• Coordinates: 11388..11546 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	11129..11323 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS29100	6209..6822	+	614	Protein_7	transposase	-
ECE24377A_RS29825	7009..7152	-	144	Protein_8	IS91 family transposase	-
ECE24377A_RS01210	7137..7502	-	366	WP_000124102.1	hypothetical protein	-
ECE24377A_RS01215	7779..8615	-	837	Protein_10	IS91 family transposase	-
ECE24377A_RS29105	8614..9912	+	1299	Protein_11	hypothetical protein	-
ECE24377A_RS01225	9967..10401	+	435	WP_000845924.1	conjugation system SOS inhibitor PsiB	-
ECE24377A_RS01230	10398..11117	+	720	WP_001276223.1	plasmid SOS inhibition protein A	-
-	11129..11323	+	195	NuclAT_0	-	Antitoxin
-	11129..11323	+	195	NuclAT_0	-	Antitoxin
-	11129..11323	+	195	NuclAT_0	-	Antitoxin
-	11129..11323	+	195	NuclAT_0	-	Antitoxin
ECE24377A_RS01235	11388..11546	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ECE24377A_RS29830	11971..12710	+	740	Protein_15	IS3 family transposase	-
ECE24377A_RS01255	13383..14891	+	1509	WP_001189123.1	group II intron reverse transcriptase/maturase	-
ECE24377A_RS01260	14981..15454	+	474	Protein_17	IS3 family transposase	-
ECE24377A_RS01265	15493..15759	-	267	Protein_18	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74224	74224
	-	inside	IScluster/Tn	-	-	1319..26700	25381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T22397 WP_001312861.1 NC_009790:11388-11546 [Escherichia coli O139:H28 str. E24377A]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T22397 NC_009790:11388-11546 [Escherichia coli O139:H28 str. E24377A]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 195 bp

>AT22397 NC_009790:11129-11323 [Escherichia coli O139:H28 str. E24377A]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGCGCAGGGATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin