Detailed information of TA system TA223887

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2672039..2672376
• Replicon: chromosome
• Accession: NZ_CP086564
• Organism: Enterococcus faecalis strain E512-TC1

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E2Z0W4
• Locus tag: LM511_RS12680
• Protein ID: WP_002381035.1
• Coordinates: 2672039..2672182 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2672114..2672376 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM511_RS12660	2667159..2667374	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LM511_RS12665	2667513..2668505	+	993	WP_002413308.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LM511_RS12670	2668769..2669407	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
LM511_RS12675	2670093..2671709	+	1617	WP_014525060.1	phosphatase PAP2/LCP family protein	-
LM511_RS12680	2672039..2672182	+	144	WP_002381035.1	putative holin-like toxin	Toxin
-	2672114..2672376	-	263	-	-	Antitoxin
LM511_RS12685	2672377..2677068	-	4692	WP_010783624.1	WxL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5202.20 Da
• Isoelectric Point: 10.0041

>T223887 WP_002381035.1 NZ_CP086564:2672039-2672182 [Enterococcus faecalis]
MNVSTKIYERRGLLSIAEALALMISFGSFIATLIFGILEAVKENNKK

Nucleotide

• Length: 144 bp

>T223887 NZ_CP119399:c1242222-1241476 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Nucleotide

• Length: 263 bp

>AT223887 NZ_CP086564:c2672376-2672114 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A2Z6BWH2

Antitoxin