Detailed information of TA system TA223853

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 746952..747184
• Replicon: chromosome
• Accession: NZ_CP086560
• Organism: Enterococcus faecalis strain E509

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: C7CXQ5
• Locus tag: LM510_RS03595
• Protein ID: WP_002355568.1
• Coordinates: 746952..747068 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	746979..747184 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM510_RS03585	742390..744648	+	2259	WP_002363179.1	DNA helicase PcrA	-
LM510_RS03590	744774..746804	+	2031	WP_002378031.1	NAD-dependent DNA ligase LigA	-
LM510_RS03595	746952..747068	+	117	WP_002355568.1	putative holin-like toxin	Toxin
-	746979..747184	-	206	-	-	Antitoxin
LM510_RS03600	747386..747691	+	306	WP_002355569.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
LM510_RS03605	747691..749160	+	1470	WP_002358701.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
LM510_RS03610	749160..750590	+	1431	WP_002358703.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
LM510_RS03615	750609..751697	+	1089	WP_002355572.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-35)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4065.93 Da
• Isoelectric Point: 5.9482

>T223853 WP_002355568.1 NZ_CP086560:746952-747068 [Enterococcus faecalis]
MFLSVEAALGLMIGFATLVVTIIFGILALVLDNKNNRS

Nucleotide

• Length: 117 bp

>T223853 NZ_CP119366:1949536-1950111 [Pseudomonas sp. CBSPBW29]
ATGGACATCGAAAAACTCAAGGGCCGCCTCGACTTCCTGCGCGAAGCGGAAAAACTCAAGGACGTGCTCAGAAGCGCGCA
CACCTCTTCCGGCCGCACCGAAAGCACCGCGGAACACAGCTGGCGCCTGTGCCTGATGGCAATTACCTTCGGGGATGAAC
TGGCCGGCCTCGACCTGCTGAAAATCCTCAAGATGTGCGTAATCCACGACCTGGGAGAGGCCCTCCACGGCGACATCCCG
GCGGTCCAGCAAGCGCAGTTCCCCAACAAGAGCCAGCAAGAACACGACGACCTGCTGTTGCTGACCCGAGCACTGGATGA
GCCGTTGCGGGTCGAGATTCTGGCCCTGTGGGACGACTACGAAAACGCCCGGTCCGCCGAGGCCAAGGCAGTCAAAGCCC
TGGACAAACTGGAGACCCTGCTGCAACACAACCAGGGCCTGAACCCGCCCGACTTCGACTACGCCTTCAATCTGGCCTAT
GGCAAGAAACACACCAGCGCCGAGCCGATTTTCCAGACACTGCGCGAGATCATCGACCAGGATACAGAAGCAAAAATCCA
GACTCCCAACCGGTAG

Antitoxin

Nucleotide

• Length: 206 bp

>AT223853 NZ_CP086560:c747184-746979 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A1B4XLM6

Antitoxin