Detailed information of TA system TA223835

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2900636..2900898
• Replicon: chromosome
• Accession: NZ_CP086558
• Organism: Enterococcus faecalis strain E512

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: S4BYM2
• Locus tag: LM505_RS14315
• Protein ID: WP_002392696.1
• Coordinates: 2900755..2900898 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2900636..2900822 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM505_RS14300	2896003..2896716	-	714	WP_010820328.1	trehalose operon repressor	-
LM505_RS14305	2896978..2899721	+	2744	Protein_2801	glycoside hydrolase family 65 protein	-
LM505_RS14310	2899736..2900386	+	651	WP_002367457.1	beta-phosphoglucomutase	-
-	2900636..2900822	+	187	-	-	Antitoxin
LM505_RS14315	2900755..2900898	-	144	WP_002392696.1	type I toxin-antitoxin system toxin PepG1	Toxin
LM505_RS14320	2901130..2902101	-	972	WP_002381060.1	ribose-phosphate diphosphokinase	-
LM505_RS14325	2902276..2902713	-	438	WP_002354871.1	peptide-methionine (R)-S-oxide reductase MsrB	-
LM505_RS14330	2902846..2903399	-	554	Protein_2806	Maf family protein	-
LM505_RS14335	2903424..2905556	-	2133	WP_010820331.1	DNA mismatch repair endonuclease MutL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5297.43 Da
• Isoelectric Point: 10.6867

>T223835 WP_002392696.1 NZ_CP086558:c2900898-2900755 [Enterococcus faecalis]
MSVLPKFTERRGLLSAYETIQTILGFGMFTIALIALIVKLLKNDNKK

Nucleotide

• Length: 144 bp

>T223835 NZ_CP119341:c917579-917391 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Nucleotide

• Length: 187 bp

>AT223835 NZ_CP086558:2900636-2900822 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: S4BYM2

Antitoxin