Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3753291..3753985 Replicon chromosome
Accession NZ_CP086556
Organism Escherichia coli strain ESY002

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag C5N07_RS18120 Protein ID WP_001263489.1
Coordinates 3753291..3753689 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag C5N07_RS18125 Protein ID WP_000554758.1
Coordinates 3753692..3753985 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3748879) 3748879..3748959 - 81 NuclAT_11 - -
- (3748879) 3748879..3748959 - 81 NuclAT_11 - -
- (3748879) 3748879..3748959 - 81 NuclAT_11 - -
- (3748879) 3748879..3748959 - 81 NuclAT_11 - -
C5N07_RS18095 (3749555) 3749555..3750013 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
C5N07_RS18100 (3750274) 3750274..3751731 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
C5N07_RS18105 (3751788) 3751788..3752309 - 522 Protein_3546 peptide chain release factor H -
C5N07_RS18110 (3752305) 3752305..3752511 - 207 Protein_3547 RtcB family protein -
C5N07_RS18115 (3752829) 3752829..3753281 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
C5N07_RS18120 (3753291) 3753291..3753689 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C5N07_RS18125 (3753692) 3753692..3753985 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C5N07_RS18130 (3754037) 3754037..3755092 - 1056 WP_001226164.1 DNA polymerase IV -
C5N07_RS18135 (3755163) 3755163..3755948 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
C5N07_RS18140 (3755920) 3755920..3757632 + 1713 Protein_3553 flagellar biosynthesis protein FlhA -
C5N07_RS18145 (3757856) 3757856..3758353 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T223811 WP_001263489.1 NZ_CP086556:c3753689-3753291 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T223811 NZ_CP119332:c4300236-4299298 [Klebsiella pneumoniae]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCAACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGTATGTTACTTGGCAGTGAAGGGATTTCGAGAGAGATTTTGGTGGGTTTTTTAACCTA
TACATTAAGTCACCCTCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTGAATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCCATGATGTTAATTGAGCTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGACTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAATTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACACCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT223811 WP_000554758.1 NZ_CP086556:c3753985-3753692 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT223811 NZ_CP119332:c4301020-4300229 [Klebsiella pneumoniae]
ATGTCATCTAAGATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
TGTGAGCTACTCCCTGGCTCAAAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTTAGTTCTGGCGTGTACTATCGTCCAG
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTAGATGTGCAATTGGGCGTTTCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTACAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAATAAGTCGTCCTTACCAAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGTGGAAACCATAAGCTTGAAG
TGAATCCCGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAACTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGACGCGATTGGGAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAGCGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTAAATTGGGGGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References