Detailed information of TA system TA223761

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42449..42713
• Replicon: plasmid pEc-042-T5-MAC_2
• Accession: NZ_CP086540
• Organism: Escherichia coli strain Ec-042-T5-MAC

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: LNN52_RS23210
• Protein ID: WP_001387489.1
• Coordinates: 42449..42601 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	42653..42713 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN52_RS23175 (38014)	38014..38130	+	117	Protein_43	IncI1-type conjugal transfer lipoprotein TraH	-
LNN52_RS23180 (38129)	38129..39883	+	1755	Protein_44	DotA/TraY family protein	-
LNN52_RS23185 (39954)	39954..40616	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
LNN52_RS23190 (40688)	40688..40897	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
LNN52_RS23195 (41289)	41289..41465	+	177	WP_001054897.1	hypothetical protein	-
LNN52_RS23600 (41530)	41530..41625	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
LNN52_RS23205 (42126)	42126..42377	+	252	WP_001291964.1	hypothetical protein	-
LNN52_RS23210 (42449)	42449..42601	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (42653)	42653..42713	+	61	NuclAT_0	-	Antitoxin
- (42653)	42653..42713	+	61	NuclAT_0	-	Antitoxin
- (42653)	42653..42713	+	61	NuclAT_0	-	Antitoxin
- (42653)	42653..42713	+	61	NuclAT_0	-	Antitoxin
LNN52_RS23215 (42922)	42922..43296	+	375	WP_223349261.1	hypothetical protein	-
LNN52_RS23220 (43449)	43449..44606	+	1158	Protein_52	IS1380-like element ISEcp1 family transposase	-
LNN52_RS23225 (44662)	44662..45359	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
LNN52_RS23230 (45370)	45370..45678	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
LNN52_RS23235 (46120)	46120..46407	-	288	WP_000074855.1	conjugal transfer protein TraA	-
LNN52_RS23240 (46445)	46445..46603	-	159	Protein_56	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..47514	47514

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T223761 WP_001387489.1 NZ_CP086540:c42601-42449 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T223761 NZ_CP119178:c181472-181002 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT223761 NZ_CP086540:42653-42713 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin