Detailed information of TA system TA223746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1109303..1110030
• Replicon: chromosome
• Accession: NZ_CP086538
• Organism: Escherichia coli strain Ec-042-T5-MAC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LNN52_RS05405
• Protein ID: WP_000547564.1
• Coordinates: 1109303..1109614 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN52_RS05410
• Protein ID: WP_000126294.1
• Coordinates: 1109611..1110030 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN52_RS05375 (1104445)	1104445..1106154	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
LNN52_RS05380 (1106164)	1106164..1106706	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
LNN52_RS05385 (1106706)	1106706..1107473	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LNN52_RS05390 (1107470)	1107470..1107880	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LNN52_RS05395 (1107873)	1107873..1108343	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LNN52_RS05400 (1108368)	1108368..1109129	+	762	WP_001026446.1	hypothetical protein	-
LNN52_RS05405 (1109303)	1109303..1109614	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LNN52_RS05410 (1109611)	1109611..1110030	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LNN52_RS05415 (1110144)	1110144..1111568	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
LNN52_RS05420 (1111577)	1111577..1113034	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LNN52_RS05425 (1113294)	1113294..1114304	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
LNN52_RS05430 (1114453)	1114453..1114980	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T223746 WP_000547564.1 NZ_CP086538:1109303-1109614 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T223746 NZ_CP119174:357250-357597 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT223746 WP_000126294.1 NZ_CP086538:1109611-1110030 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT223746 NZ_CP119174:357597-357896 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5