Detailed information of TA system TA223720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1109304..1110031
• Replicon: chromosome
• Accession: NZ_CP086532
• Organism: Escherichia coli strain Ec-042-T5-ESBL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LNN41_RS05400
• Protein ID: WP_000547564.1
• Coordinates: 1109304..1109615 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN41_RS05405
• Protein ID: WP_000126294.1
• Coordinates: 1109612..1110031 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN41_RS05370 (1104446)	1104446..1106155	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
LNN41_RS05375 (1106165)	1106165..1106707	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
LNN41_RS05380 (1106707)	1106707..1107474	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LNN41_RS05385 (1107471)	1107471..1107881	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LNN41_RS05390 (1107874)	1107874..1108344	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LNN41_RS05395 (1108369)	1108369..1109130	+	762	WP_001026446.1	hypothetical protein	-
LNN41_RS05400 (1109304)	1109304..1109615	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LNN41_RS05405 (1109612)	1109612..1110031	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LNN41_RS05410 (1110145)	1110145..1111569	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
LNN41_RS05415 (1111578)	1111578..1113035	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LNN41_RS05420 (1113295)	1113295..1114305	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
LNN41_RS05425 (1114454)	1114454..1114981	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T223720 WP_000547564.1 NZ_CP086532:1109304-1109615 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T223720 NZ_CP119159:c320438-320334 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
GCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT223720 WP_000126294.1 NZ_CP086532:1109612-1110031 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT223720 NZ_CP119159:320146-320401 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCAGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAATGA
TAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATTTTATTATTTAAA
AATAACCGTGCTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAAGCA
ATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5