Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 653745..654544 Replicon chromosome
Accession NZ_CP086532
Organism Escherichia coli strain Ec-042-T5-ESBL

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LNN41_RS03190 Protein ID WP_000347251.1
Coordinates 653745..654209 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LNN41_RS03195 Protein ID WP_001307405.1
Coordinates 654209..654544 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN41_RS03160 (648746) 648746..649180 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LNN41_RS03165 (649198) 649198..650076 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LNN41_RS03170 (650066) 650066..650845 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LNN41_RS03175 (650856) 650856..651329 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LNN41_RS03180 (651352) 651352..652632 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
LNN41_RS03185 (652881) 652881..653690 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LNN41_RS03190 (653745) 653745..654209 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LNN41_RS03195 (654209) 654209..654544 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LNN41_RS03200 (654693) 654693..656264 - 1572 WP_001273761.1 galactarate dehydratase -
LNN41_RS03205 (656639) 656639..657973 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
LNN41_RS03210 (657989) 657989..658759 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T223716 WP_000347251.1 NZ_CP086532:c654209-653745 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T223716 NZ_CP119151:7690-8040 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223716 WP_001307405.1 NZ_CP086532:c654544-654209 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223716 NZ_CP119151:7433-7696 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References