Detailed information of TA system TA022371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3817187..3817788
• Replicon: chromosome
• Accession: NC_009778
• Organism: Cronobacter sakazakii ATCC BAA-894

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6L3P9G2
• Locus tag: ESA_RS17830
• Protein ID: WP_012126103.1
• Coordinates: 3817477..3817788 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ESA_RS17825
• Protein ID: WP_007871962.1
• Coordinates: 3817187..3817477 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ESA_RS17810	3814653..3815555	+	903	WP_004385164.1	formate dehydrogenase subunit beta	-
ESA_RS17815	3815552..3816187	+	636	WP_004385165.1	formate dehydrogenase cytochrome b556 subunit	-
ESA_RS17820	3816184..3817113	+	930	WP_007871963.1	formate dehydrogenase accessory protein FdhE	-
ESA_RS17825	3817187..3817477	-	291	WP_007871962.1	helix-turn-helix domain-containing protein	Antitoxin
ESA_RS17830	3817477..3817788	-	312	WP_012126103.1	hypothetical protein	Toxin
ESA_RS17835	3817963..3818787	-	825	WP_012126104.1	AraC family transcriptional regulator	-
ESA_RS17840	3819048..3820451	+	1404	WP_012126105.1	MFS transporter	-
ESA_RS17845	3820463..3822430	+	1968	WP_012126106.1	glycoside hydrolase family 127 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12070.99 Da
• Isoelectric Point: 10.0150

>T22371 WP_012126103.1 NC_009778:c3817788-3817477 [Cronobacter sakazakii ATCC BAA-894]
MLFIETEIFTRAVTALLSDDDYQRLQWFLALRPDYGDVIPDTGGLRKARWLSGGRGKRGGVRIIYFYRPAADEIRLLLIY
RKGIKDDLSPHEKSVLRMLNERW

Nucleotide

• Length: 312 bp

>T22371 NC_009778:c3817788-3817477 [Cronobacter sakazakii ATCC BAA-894]
ATGCTCTTCATTGAAACGGAAATCTTTACCCGGGCCGTGACTGCCCTGCTTAGCGATGACGATTACCAGCGGTTGCAGTG
GTTCCTGGCCCTGCGGCCTGATTACGGCGACGTGATACCGGATACCGGCGGGCTTCGTAAGGCGCGCTGGCTGTCAGGCG
GCCGGGGAAAACGGGGCGGCGTCAGGATCATCTATTTTTATCGCCCTGCTGCCGATGAAATCCGCCTGCTGCTGATTTAT
CGCAAGGGGATAAAAGACGATCTCTCGCCTCACGAAAAATCCGTGCTCAGAATGCTGAATGAGAGGTGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10659.23 Da
• Isoelectric Point: 10.7279

>AT22371 WP_007871962.1 NC_009778:c3817477-3817187 [Cronobacter sakazakii ATCC BAA-894]
MDKALFEQLTESLRQMNDISEGRREPSRVFVIDALKIKAIREASGLSQTKFASLISVSVDTLRNWEQGRRSPTGPARALL
RAIANDPQHVLSALAL

Nucleotide

• Length: 291 bp

>AT22371 NC_009778:c3817477-3817187 [Cronobacter sakazakii ATCC BAA-894]
ATGGACAAGGCGCTGTTTGAACAGTTAACCGAAAGCCTGCGGCAGATGAATGATATCAGCGAAGGCCGCCGAGAGCCCTC
ACGGGTTTTTGTTATCGACGCGCTGAAAATAAAAGCGATCAGAGAGGCATCAGGCCTTTCTCAGACGAAATTCGCCAGCC
TGATTTCAGTAAGTGTGGATACGCTGCGTAACTGGGAGCAGGGAAGGCGCTCGCCGACCGGGCCTGCGCGCGCGCTGTTG
CGTGCGATTGCGAACGATCCGCAGCATGTTCTCTCTGCGCTGGCGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A6L3P9G2