Detailed information of TA system TA223694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1109316..1110043
• Replicon: chromosome
• Accession: NZ_CP086526
• Organism: Escherichia coli strain Ec-042-T20-MAC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LNN58_RS05410
• Protein ID: WP_000547564.1
• Coordinates: 1109316..1109627 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN58_RS05415
• Protein ID: WP_000126294.1
• Coordinates: 1109624..1110043 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN58_RS05380 (1104458)	1104458..1106167	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
LNN58_RS05385 (1106177)	1106177..1106719	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
LNN58_RS05390 (1106719)	1106719..1107486	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LNN58_RS05395 (1107483)	1107483..1107893	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LNN58_RS05400 (1107886)	1107886..1108356	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LNN58_RS05405 (1108381)	1108381..1109142	+	762	WP_001026446.1	hypothetical protein	-
LNN58_RS05410 (1109316)	1109316..1109627	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LNN58_RS05415 (1109624)	1109624..1110043	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LNN58_RS05420 (1110157)	1110157..1111581	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
LNN58_RS05425 (1111590)	1111590..1113047	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LNN58_RS05430 (1113307)	1113307..1114317	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
LNN58_RS05435 (1114466)	1114466..1114993	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T223694 WP_000547564.1 NZ_CP086526:1109316-1109627 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T223694 NZ_CP119137:324121-324489 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT223694 WP_000126294.1 NZ_CP086526:1109624-1110043 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT223694 NZ_CP119137:323903-324124 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTAACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5