Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 653757..654556 Replicon chromosome
Accession NZ_CP086526
Organism Escherichia coli strain Ec-042-T20-MAC

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LNN58_RS03200 Protein ID WP_000347251.1
Coordinates 653757..654221 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LNN58_RS03205 Protein ID WP_001307405.1
Coordinates 654221..654556 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN58_RS03170 (648758) 648758..649192 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LNN58_RS03175 (649210) 649210..650088 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LNN58_RS03180 (650078) 650078..650857 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LNN58_RS03185 (650868) 650868..651341 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LNN58_RS03190 (651364) 651364..652644 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
LNN58_RS03195 (652893) 652893..653702 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LNN58_RS03200 (653757) 653757..654221 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LNN58_RS03205 (654221) 654221..654556 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LNN58_RS03210 (654705) 654705..656276 - 1572 WP_001273761.1 galactarate dehydratase -
LNN58_RS03215 (656651) 656651..657985 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
LNN58_RS03220 (658001) 658001..658771 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T223690 WP_000347251.1 NZ_CP086526:c654221-653757 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T223690 NZ_CP119131:649-936 [Sphingobium sp. WTD-1]
ATGAAGGCCCTTTCCTTCTCGCCTCGGGCAGAAGTCGACATTGACGGTATCTGGGACTACAGCGCCGACAACTGGGGGCC
GGATCAGGCTGACCGCTACACCGACGAAATCCGGGATGCCTGCCATGCGCTGGCATCGGGCCGGAAGCAGGGGCGGGCGA
CGAATGTTCGCCTTGGCTATCGCAAATGCCCCACCGGCTCGCATGTGATCTATTTCCGCGACCGGGGGGACTGGCTGGAG
ATTGTCCGCGTCCTGCATAGCGCACAGGACGCGGAGCGGCACCTGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223690 WP_001307405.1 NZ_CP086526:c654556-654221 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223690 NZ_CP119131:404-652 [Sphingobium sp. WTD-1]
ATGGCCCGCAATACATCCGTGTCCCTCGGGGACCATTTCACCACGTTCATCGACATGCAGGTGCAGGCCGGGCGCTACGG
ATCGGCCAGCGATGTGGTGCGGGCAGGGCTGCGGCTGCTGCAAGAGCATGAGGCCAAGGTGAAGGCGCTGGAAGTCGCCC
TGATCGAGGGCGAGGAATCCGGCGAACCGCAGCCCTTTGACTTCGAAGCGTTCAAGAAGCGGAAGCGGGCGGAGTATGAG
GGCAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References