Detailed information of TA system TA223683

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42128..42392
• Replicon: plasmid pEc-042-T20-ESBL_2
• Accession: NZ_CP086522
• Organism: Escherichia coli strain Ec-042-T20-ESBL

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: LNN45_RS23180
• Protein ID: WP_001387489.1
• Coordinates: 42128..42280 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	42332..42392 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN45_RS23145 (37693)	37693..37809	+	117	Protein_43	IncI1-type conjugal transfer lipoprotein TraH	-
LNN45_RS23150 (37808)	37808..39562	+	1755	Protein_44	DotA/TraY family protein	-
LNN45_RS23155 (39633)	39633..40295	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
LNN45_RS23160 (40367)	40367..40576	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
LNN45_RS23165 (40968)	40968..41144	+	177	WP_001054897.1	hypothetical protein	-
LNN45_RS23565 (41209)	41209..41304	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
LNN45_RS23175 (41805)	41805..42056	+	252	WP_001291964.1	hypothetical protein	-
LNN45_RS23180 (42128)	42128..42280	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (42332)	42332..42392	+	61	NuclAT_0	-	Antitoxin
- (42332)	42332..42392	+	61	NuclAT_0	-	Antitoxin
- (42332)	42332..42392	+	61	NuclAT_0	-	Antitoxin
- (42332)	42332..42392	+	61	NuclAT_0	-	Antitoxin
LNN45_RS23185 (42601)	42601..42975	+	375	WP_223349261.1	hypothetical protein	-
LNN45_RS23190 (43128)	43128..44285	+	1158	Protein_52	IS1380-like element ISEcp1 family transposase	-
LNN45_RS23195 (44341)	44341..45038	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
LNN45_RS23200 (45049)	45049..45357	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
LNN45_RS23205 (45799)	45799..46086	-	288	WP_000074855.1	conjugal transfer protein TraA	-
LNN45_RS23210 (46124)	46124..46282	-	159	Protein_56	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..47193	47193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T223683 WP_001387489.1 NZ_CP086522:c42280-42128 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T223683 NZ_CP119127:3774189-3774467 [Sphingobium sp. WTD-1]
ATGACCCTGCCGGTCGTCTGGGATGACGAGGCCGAAACAGCCCTCCTCACCATCCTCGACTATATCGGCCCGCGCAACAA
AACTGCGGCCGAGCGCCTCTATGCCGCCATCCGGCACACTGCCGACAATCTGCCCAACCACCCCTATGCGCATCGCCCAG
GCCGCACGCCCGGCACGCGCGAAGCCGTGGTTCACCCCAATTATATCCTCATCTACCGCGTCGGGATCGAGGCGATCGAG
ATATTGACGCTCGTTCACGCGCGCCAGCAATATCCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT223683 NZ_CP086522:42332-42392 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin