Detailed information of TA system TA223656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 67561..68272
• Replicon: plasmid pEc-050-T0_3
• Accession: NZ_CP086513
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: LNN50_RS24955
• Protein ID: WP_000162415.1
• Coordinates: 67970..68272 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN50_RS24950
• Protein ID: WP_000806445.1
• Coordinates: 67561..67899 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN50_RS24920 (LNN50_24925)	62856..63677	-	822	WP_001427910.1	hypothetical protein	-
LNN50_RS24925 (LNN50_24930)	64023..64676	+	654	WP_000410952.1	hypothetical protein	-
LNN50_RS24930 (LNN50_24935)	65005..65334	+	330	WP_000542383.1	hypothetical protein	-
LNN50_RS24935 (LNN50_24940)	65327..66520	+	1194	WP_102218742.1	phage tail protein	-
LNN50_RS24940 (LNN50_24945)	66554..67282	-	729	WP_000986754.1	hypothetical protein	-
LNN50_RS24945 (LNN50_24950)	67319..67504	-	186	Protein_82	hypothetical protein	-
LNN50_RS24950 (LNN50_24955)	67561..67899	-	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
LNN50_RS24955 (LNN50_24960)	67970..68272	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN50_RS24960 (LNN50_24965)	68435..69226	+	792	WP_000532305.1	hypothetical protein	-
LNN50_RS24965 (LNN50_24970)	69223..69990	+	768	WP_000203290.1	hypothetical protein	-
LNN50_RS24970 (LNN50_24975)	69994..70974	+	981	WP_000046499.1	hypothetical protein	-
LNN50_RS24975 (LNN50_24980)	70971..71624	+	654	WP_000828873.1	hypothetical protein	-
LNN50_RS24980 (LNN50_24985)	71684..72589	+	906	WP_001258014.1	recombination-associated protein RdgC	-
LNN50_RS24985 (LNN50_24990)	72603..73253	+	651	WP_032153356.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90446	90446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T223656 WP_000162415.1 NZ_CP086513:c68272-67970 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T223656 NZ_CP119120:443305-443478 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT223656 WP_000806445.1 NZ_CP086513:c67899-67561 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT223656 NZ_CP119120:442981-443277 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A631VBQ3