Detailed information of TA system TA223644

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53246..53510
• Replicon: plasmid pEc-050-T0_1
• Accession: NZ_CP086511
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: LNN50_RS23575
• Protein ID: WP_001303307.1
• Coordinates: 53358..53510 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	53246..53308 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN50_RS23560 (48485)	48485..50776	-	2292	WP_001289275.1	F-type conjugative transfer protein TrbC	-
LNN50_RS23565 (50769)	50769..51839	-	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
LNN50_RS23570 (51858)	51858..53066	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (53246)	53246..53308	-	63	NuclAT_0	-	Antitoxin
- (53246)	53246..53308	-	63	NuclAT_0	-	Antitoxin
- (53246)	53246..53308	-	63	NuclAT_0	-	Antitoxin
- (53246)	53246..53308	-	63	NuclAT_0	-	Antitoxin
LNN50_RS23575 (53358)	53358..53510	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
LNN50_RS23580 (53582)	53582..53833	-	252	WP_001291965.1	hypothetical protein	-
LNN50_RS26385 (54333)	54333..54428	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
LNN50_RS23590 (54493)	54493..54630	-	138	WP_047088497.1	hypothetical protein	-
LNN50_RS23595 (54675)	54675..55901	-	1227	WP_024186316.1	RNA-guided endonuclease TnpB family protein	-
LNN50_RS23600 (55960)	55960..56364	+	405	WP_001175009.1	IS200/IS605 family transposase	-
LNN50_RS23605 (56696)	56696..56905	-	210	WP_032178830.1	hemolysin expression modulator Hha	-
LNN50_RS23610 (57003)	57003..57665	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / floR / blaTEM-30 / sul1 / qacE / ant(3'')-Ia / dfrA1	-	1..115932	115932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T223644 WP_001303307.1 NZ_CP086511:53358-53510 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T223644 NZ_CP119109:786875-787237 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 63 bp

>AT223644 NZ_CP086511:c53308-53246 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin