Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3792419..3792960 Replicon chromosome
Accession NZ_CP086510
Organism Escherichia coli strain Ec-050-T0

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LNN50_RS18260 Protein ID WP_000615983.1
Coordinates 3792682..3792960 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LNN50_RS18255 Protein ID WP_000729703.1
Coordinates 3792419..3792679 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN50_RS18235 (3788094) 3788094..3788879 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LNN50_RS18240 (3788851) 3788851..3790563 + 1713 Protein_3594 flagellar biosynthesis protein FlhA -
LNN50_RS18245 (3790787) 3790787..3791284 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LNN50_RS18250 (3791460) 3791460..3792209 - 750 WP_000056849.1 C40 family peptidase -
LNN50_RS18255 (3792419) 3792419..3792679 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LNN50_RS18260 (3792682) 3792682..3792960 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LNN50_RS18265 (3793116) 3793116..3793856 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LNN50_RS18270 (3793827) 3793827..3794594 - 768 WP_000333380.1 class II glutamine amidotransferase -
LNN50_RS18275 (3794800) 3794800..3795378 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T223640 WP_000615983.1 NZ_CP086510:3792682-3792960 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T223640 NZ_CP119108:3576290-3576850 [Microbacterium sp. KACC 23027]
GTGACGGGCCTCGATGAGGAACTGGTCCAACACGTCGACCAGCTGGGAAGCGCTGGCTGGACCTCCACAACCCACCGCTA
CACGGCGGCACGCCGCGACCCACTCTCGGGAGCAGGCGCCCGGTTGTTCGGCGGACGTTGGAATCCAAAGGACATCTTCG
CGACGATCTACCTGGCACAGCCGCTCGCCGCATGTCTTGGTGAAGTGGAGCGCACTGCGAAAAGCCAAAGCACCACCCCC
GACGTCCTGCTTCGCGCCAGCTACACACTGCACGAGATCAACGTCACCAACCTGCCCGTCCTTGACCTCCGCGATGAGCA
TGCGCTCGCACACGTCGGCCTGACGCTCGCTGACATCTCCGACCCTGACTGGACCGCCTGCCAAGCGGTGGGCAACGCCG
CCTGGTTCCTTGGCCTCGGTGGCGTGCTCGCACCCTCCGCTACCGAAGAGGGTGTCGTGCTCGCAGCGTTTGAGGCCCGC
ATCGTTGGGCACTTGACCGTGGCCAAGAGCGAGCCGCTCACCCCTGAACTGTTCGAGACGCTCCGAGCCGGGACGGCATA
G

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT223640 WP_000729703.1 NZ_CP086510:3792419-3792679 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT223640 NZ_CP119108:3575940-3576293 [Microbacterium sp. KACC 23027]
ATGAGCACAGCCGTGGCACGCGGTGCGTTGGCCCAAAAGGTGGCCGACATCATCGATCACCTCGGGCTGACGCAGGAGGA
AGTCGGAGAGATCGTTGACGCGTCGGCACGCTCCGTCGCCCGCTGGTCAAGCGGCGAAGTCGTACCCCAACGACTCAACA
AGCAACGGCTTCTTGAACTCGCGTACGTTGCCCAAGCCGTCGCCGAGATACTCCCCGCCGACAATGCGAACGTCTGGATC
CACACTCCGAACCGACTCCTGGCGCACGACACGCCCGCCCAGCGCATCCACGCTGGCGCCTATCGAGACGTGCTAAACCT
GATCGAGGCCATCGCCGAAGGCATCGTGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References