Detailed information of TA system TA223639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3786222..3786916
• Replicon: chromosome
• Accession: NZ_CP086510
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LNN50_RS18220
• Protein ID: WP_001263489.1
• Coordinates: 3786222..3786620 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LNN50_RS18225
• Protein ID: WP_000554758.1
• Coordinates: 3786623..3786916 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3781810)	3781810..3781890	-	81	NuclAT_12	-	-
- (3781810)	3781810..3781890	-	81	NuclAT_12	-	-
- (3781810)	3781810..3781890	-	81	NuclAT_12	-	-
- (3781810)	3781810..3781890	-	81	NuclAT_12	-	-
LNN50_RS18195 (3782486)	3782486..3782944	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LNN50_RS18200 (3783205)	3783205..3784662	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LNN50_RS18205 (3784719)	3784719..3785240	-	522	Protein_3587	peptide chain release factor H	-
LNN50_RS18210 (3785236)	3785236..3785442	-	207	Protein_3588	RtcB family protein	-
LNN50_RS18215 (3785760)	3785760..3786212	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LNN50_RS18220 (3786222)	3786222..3786620	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LNN50_RS18225 (3786623)	3786623..3786916	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LNN50_RS18230 (3786968)	3786968..3788023	-	1056	WP_001226164.1	DNA polymerase IV	-
LNN50_RS18235 (3788094)	3788094..3788879	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LNN50_RS18240 (3788851)	3788851..3790563	+	1713	Protein_3594	flagellar biosynthesis protein FlhA	-
LNN50_RS18245 (3790787)	3790787..3791284	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T223639 WP_001263489.1 NZ_CP086510:c3786620-3786222 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T223639 NZ_CP119108:c3482445-3482077 [Microbacterium sp. KACC 23027]
GTGAGCGGCGAGCTGCCCTTCGTGCGGCGCGGCGCCATTGTGCTCGTCGACCTCGATCCGTCTGACGGGTCTGAGGCTGC
CAAGCTCCGACCCGCCGTCGTCGTGAGCAACAACACCGCGAACGCCGCCGCGCTCCGCACCGGCCGCGGCGTGCTCACCG
TGGTGCCGGTCACTTCGAACACGGAGAAGGTCTATCCGTTCCAGGTGCTGCTGCCGGCGGCATCCACCGGTCTCTCCCGT
GACTCGAAGGCGCAGGCCGAACAGCTCCGCGCGGTCTCGCTGCTGCGCGTGCGTCGGGTCGTCGGATGGGTGCCGGGCGA
CCTTATGGCCGGCCTCGACGCGGCGGTGCGTCTGCATCTGGCGTTGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT223639 WP_000554758.1 NZ_CP086510:c3786916-3786623 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT223639 NZ_CP119108:c3482669-3482442 [Microbacterium sp. KACC 23027]
ATGGTCTCGAAGGTCAGCATCAGTCTTGACGACGACGACGTCGCGTACCTCGACGGGCAGTCGCTCTCCGGGCGCTTCGC
CAGCCGGTCGGCGGCGGTGCAGGCGGCCATCCGGCTCCTGCGCGAGAGTGAGCTCTCCGATGCCTACGCTGAGGCGTACG
GCTCATGGGACGAGTCCGACGACGCGGCGCCCTGGGATGCTACGGCCGCCGACGGGCTCCCCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3