Detailed information of TA system TA223626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1907581..1908083
• Replicon: chromosome
• Accession: NZ_CP086510
• Organism: Escherichia coli strain Ec-050-T0

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: LNN50_RS09120
• Protein ID: WP_000767829.1
• Coordinates: 1907829..1908083 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LNN50_RS09115
• Protein ID: WP_001259255.1
• Coordinates: 1907581..1907832 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN50_RS09090 (1902758)	1902758..1903825	-	1068	WP_000080090.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
LNN50_RS09095 (1903825)	1903825..1904895	-	1071	WP_000108933.1	histidinol-phosphate transaminase	-
LNN50_RS09100 (1904892)	1904892..1906196	-	1305	WP_074514295.1	histidinol dehydrogenase	-
LNN50_RS09105 (1906202)	1906202..1907101	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LNN50_RS09110 (1907247)	1907247..1907297	-	51	WP_001364200.1	his operon leader peptide	-
LNN50_RS09115 (1907581)	1907581..1907832	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LNN50_RS09120 (1907829)	1907829..1908083	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LNN50_RS09125 (1908166)	1908166..1908990	+	825	WP_000754737.1	SDR family oxidoreductase	-
LNN50_RS09130 (1909036)	1909036..1909965	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
LNN50_RS09135 (1910180)	1910180..1910242	+	63	WP_010723108.1	membrane protein YoeI	-
LNN50_RS09140 (1910232)	1910232..1911590	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
LNN50_RS09145 (1911769)	1911769..1912827	+	1059	WP_087523589.1	thiosulfate utilization transporter TsuA/YeeE	-
LNN50_RS09150 (1912841)	1912841..1913068	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T223626 WP_000767829.1 NZ_CP086510:1907829-1908083 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T223626 NZ_CP119076:3726376-3726594 [Klebsiella aerogenes]
ATGTCTGGTAAACCATTAACTAAAACAGACTATTTGATGCGTTTACGCCGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAGAATAAATACGAACTTTCCGACAATGAACTGGCGGTATTTTACTCCGCCGCTGACCATCGTCTTGCTGAAT
TGACCATGAATAAGCTATACGACAAAATCCCCACTTCAGTATGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT223626 WP_001259255.1 NZ_CP086510:1907581-1907832 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT223626 NZ_CP119076:3725975-3726349 [Klebsiella aerogenes]
ATGGACGAGTACTCACCAAAAAGGCATGATATCGCGCAGCTGAGATTCCTCTGCGAAACTCTGTATCATGACTGCCTTGC
AAACCTGGAAGAAAGCAACCGCGGCTGGGTCAACGATCCAACCTCGGCAGTGAATCTTCAAATAAATGAATTGATCGAGC
ACATAGCAACTTTCGCGCTTAATTATAAAATTAAGTACGCTGAGGATAATAAACTGATTTCCCAGCTTGACGAATACTTG
GATGACACTTTTATGCTGTTCAGTAGCTATGGCATAAACACAAGCGATCTACAGAAATGGCGCAAATCCGGCAATCGGTT
ATTCCGTTGCTTCGTGAGCGCTAGCCGGGAGAATCCGGCAAGTCTTTCTTGTTAG

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0