Detailed information of TA system TA223613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 22067..22778
• Replicon: plasmid pEc-050-T5-MAC_3
• Accession: NZ_CP086503
• Organism: Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: LNN49_RS24665
• Protein ID: WP_000162415.1
• Coordinates: 22067..22369 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN49_RS24670
• Protein ID: WP_000806445.1
• Coordinates: 22440..22778 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN49_RS24635 (LNN49_24640)	17086..17736	-	651	WP_032153356.1	hypothetical protein	-
LNN49_RS24640 (LNN49_24645)	17750..18655	-	906	WP_001258014.1	recombination-associated protein RdgC	-
LNN49_RS24645 (LNN49_24650)	18715..19368	-	654	WP_000828873.1	hypothetical protein	-
LNN49_RS24650 (LNN49_24655)	19365..20345	-	981	WP_000046499.1	hypothetical protein	-
LNN49_RS24655 (LNN49_24660)	20349..21116	-	768	WP_000203290.1	hypothetical protein	-
LNN49_RS24660 (LNN49_24665)	21113..21904	-	792	WP_000532305.1	hypothetical protein	-
LNN49_RS24665 (LNN49_24670)	22067..22369	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN49_RS24670 (LNN49_24675)	22440..22778	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
LNN49_RS24675 (LNN49_24680)	22835..23020	+	186	Protein_29	hypothetical protein	-
LNN49_RS24680 (LNN49_24685)	23057..23785	+	729	WP_000986754.1	hypothetical protein	-
LNN49_RS24685 (LNN49_24690)	23819..25012	-	1194	WP_102218742.1	phage tail protein	-
LNN49_RS24690 (LNN49_24695)	25005..25334	-	330	WP_000542383.1	hypothetical protein	-
LNN49_RS24695 (LNN49_24700)	25663..26316	-	654	WP_000410952.1	hypothetical protein	-
LNN49_RS24700 (LNN49_24705)	26662..27483	+	822	WP_001427910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90296	90296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T223613 WP_000162415.1 NZ_CP086503:22067-22369 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T223613 NZ_CP119013:c5418821-5418438 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT223613 WP_000806445.1 NZ_CP086503:22440-22778 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT223613 NZ_CP119013:c5419063-5418821 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3