Detailed information of TA system TA223603

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69612..69876
• Replicon: plasmid pEc-050-T5-MAC_1
• Accession: NZ_CP086501
• Organism: Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: LNN49_RS23680
• Protein ID: WP_001303307.1
• Coordinates: 69724..69876 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	69612..69674 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN49_RS23665 (64851)	64851..67142	-	2292	WP_001289275.1	F-type conjugative transfer protein TrbC	-
LNN49_RS23670 (67135)	67135..68205	-	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
LNN49_RS23675 (68224)	68224..69432	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (69612)	69612..69674	-	63	NuclAT_0	-	Antitoxin
- (69612)	69612..69674	-	63	NuclAT_0	-	Antitoxin
- (69612)	69612..69674	-	63	NuclAT_0	-	Antitoxin
- (69612)	69612..69674	-	63	NuclAT_0	-	Antitoxin
LNN49_RS23680 (69724)	69724..69876	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
LNN49_RS23685 (69948)	69948..70199	-	252	WP_001291965.1	hypothetical protein	-
LNN49_RS26400 (70699)	70699..70794	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
LNN49_RS23695 (70859)	70859..70996	-	138	WP_047088497.1	hypothetical protein	-
LNN49_RS23700 (71041)	71041..72267	-	1227	WP_024186316.1	RNA-guided endonuclease TnpB family protein	-
LNN49_RS23705 (72326)	72326..72730	+	405	WP_001175009.1	IS200/IS605 family transposase	-
LNN49_RS23710 (73062)	73062..73271	-	210	WP_032178830.1	hemolysin expression modulator Hha	-
LNN49_RS23715 (73369)	73369..74031	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / floR / blaTEM-30 / sul1 / qacE / ant(3'')-Ia / dfrA1	-	1..117620	117620

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T223603 WP_001303307.1 NZ_CP086501:69724-69876 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T223603 NZ_CP119013:329184-329552 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT223603 NZ_CP086501:c69674-69612 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin