Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3792421..3792962 Replicon chromosome
Accession NZ_CP086500
Organism Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LNN49_RS18260 Protein ID WP_000615983.1
Coordinates 3792684..3792962 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LNN49_RS18255 Protein ID WP_000729703.1
Coordinates 3792421..3792681 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN49_RS18235 (3788096) 3788096..3788881 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LNN49_RS18240 (3788853) 3788853..3790565 + 1713 Protein_3592 flagellar biosynthesis protein FlhA -
LNN49_RS18245 (3790789) 3790789..3791286 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LNN49_RS18250 (3791462) 3791462..3792211 - 750 WP_000056849.1 C40 family peptidase -
LNN49_RS18255 (3792421) 3792421..3792681 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LNN49_RS18260 (3792684) 3792684..3792962 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LNN49_RS18265 (3793118) 3793118..3793858 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LNN49_RS18270 (3793829) 3793829..3794596 - 768 WP_000333380.1 class II glutamine amidotransferase -
LNN49_RS18275 (3794802) 3794802..3795380 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T223598 WP_000615983.1 NZ_CP086500:3792684-3792962 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T223598 NZ_CP118992:23951-24259 [Aeromonas allosaccharophila]
ATGCAGTTCACTATCTACCGCAACAAGGGCAATGCCCGCGCTTACCCCTATTTGCTGGATGTGCAAAGCGACATCATTGG
CGAGCTGGCCACCAGGATGGTGATCCCACTGTTCCCGCTGGTAGCCTTCACTGGACGGCCAGCGCAGCGGCTGAATCCGG
TGATCATCGTTGAGGGTGATAAGTATTTGGTGATGACGCAGGACATGGCAGCGGTGAGGCTGGCGCAGCTTGGGGATGAA
GTCACTGGTGTGCAGGAGTCGCGTCAGCTCATCAAGGACGCGATAGATTTCTTGTTTGATGGTGTGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT223598 WP_000729703.1 NZ_CP086500:3792421-3792681 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT223598 NZ_CP118992:23712-23951 [Aeromonas allosaccharophila]
ATGGTCGCAGCAGCAATGCGCAGCAAGAGAACGGTCAGCGTCACGCTGGAGCCCAGCTTGTTGGAACGAGCCAGAGCGGC
GGGGATCAACCTGTCAGCCACCCTTGCAACCGCGCTACAGAATGAAATCAGCGGCTCAGAAGCAGAACGCTGGAAGCGTG
AGAACAGTGCAGCCATACAGACGCTGAACCGCATCACCGAGGAGCACGGCCTGCTCTCCGACGATTACAGGGCGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References