Detailed information of TA system TA223597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3786224..3786918
• Replicon: chromosome
• Accession: NZ_CP086500
• Organism: Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LNN49_RS18220
• Protein ID: WP_001263489.1
• Coordinates: 3786224..3786622 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LNN49_RS18225
• Protein ID: WP_000554758.1
• Coordinates: 3786625..3786918 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3781812)	3781812..3781892	-	81	NuclAT_12	-	-
- (3781812)	3781812..3781892	-	81	NuclAT_12	-	-
- (3781812)	3781812..3781892	-	81	NuclAT_12	-	-
- (3781812)	3781812..3781892	-	81	NuclAT_12	-	-
LNN49_RS18195 (3782488)	3782488..3782946	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LNN49_RS18200 (3783207)	3783207..3784664	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LNN49_RS18205 (3784721)	3784721..3785242	-	522	Protein_3585	peptide chain release factor H	-
LNN49_RS18210 (3785238)	3785238..3785444	-	207	Protein_3586	RtcB family protein	-
LNN49_RS18215 (3785762)	3785762..3786214	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LNN49_RS18220 (3786224)	3786224..3786622	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LNN49_RS18225 (3786625)	3786625..3786918	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LNN49_RS18230 (3786970)	3786970..3788025	-	1056	WP_001226164.1	DNA polymerase IV	-
LNN49_RS18235 (3788096)	3788096..3788881	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LNN49_RS18240 (3788853)	3788853..3790565	+	1713	Protein_3592	flagellar biosynthesis protein FlhA	-
LNN49_RS18245 (3790789)	3790789..3791286	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T223597 WP_001263489.1 NZ_CP086500:c3786622-3786224 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T223597 NZ_CP118991:3873-4181 [Aeromonas allosaccharophila]
ATGCAGTTCACGATCTATCGCAACAAGGGCAATGCCCGCGATTATCCCTACTTGCTGAATGTGCAAAGCGACATCATCGA
CGAACTGAACACTAGGATGGTGATCCCGTTGGTACCGCTGGCGGCCTTCATTGGGCGGCCAGCGCAACGACTAAACCCCG
TTATTTCCGTCGAAGGAAAAAAGCATCTGGTGTTGACCCAAGAAATGGCGTCGGTTCGGTTGGGGCAGCTTGGAGAGGAA
GTCACTGACGTGCAGGAGTCTCGTCAGCTCATCAAGGACTCAATAGATTTCCTACTGGATGGAGTGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT223597 WP_000554758.1 NZ_CP086500:c3786918-3786625 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT223597 NZ_CP118991:3634-3873 [Aeromonas allosaccharophila]
ATGGTCGCAGCAGCAATGCGCAGCAAGAGAACGGTCAGCGTCACGCTGGAGCCCAGCTTGCTGGAACGAGCCAGAGCGGC
GGGGATCAACCTGTCTGCTACCCTAGCCACCGCGCTACAGCATGAAATCAGCGGCTCAGAGGCCGAGCGTTGGAAGCGAG
ATAACAGGGAGGCAATCCAAACCCTTAATCGGATGGTCGAAGAGCGCGGCCTGTTTTCTGACGATCACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3