Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611444..612243 Replicon chromosome
Accession NZ_CP086500
Organism Escherichia coli strain Ec-050-T5-MAC

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag LNN49_RS02970 Protein ID WP_000347275.1
Coordinates 611444..611908 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LNN49_RS02975 Protein ID WP_001307405.1
Coordinates 611908..612243 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN49_RS02940 (606445) 606445..606879 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LNN49_RS02945 (606897) 606897..607775 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LNN49_RS02950 (607765) 607765..608544 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LNN49_RS02955 (608555) 608555..609028 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LNN49_RS02960 (609051) 609051..610331 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LNN49_RS02965 (610580) 610580..611389 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LNN49_RS02970 (611444) 611444..611908 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LNN49_RS02975 (611908) 611908..612243 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LNN49_RS02980 (612392) 612392..613963 - 1572 WP_001273752.1 galactarate dehydratase -
LNN49_RS02985 (614338) 614338..615672 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LNN49_RS02990 (615688) 615688..616458 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T223578 WP_000347275.1 NZ_CP086500:c611908-611444 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T223578 NZ_CP118973:811366-811728 [Staphylococcus warneri]
ATGATTAGAAGAGGAGATGTTTATCTAGCAGATTTATCACCTGTTCAAGGGTCAGAACAAGGGGGAGTAAGACCCGTCGT
CATTATCCAAAATGACACAGGTAATAAATATAGTCCTACTGTTATTGTTGCCGCAATCACTGGAAGGATTAATAAAGCTA
AGATACCTACACATGTTGAAATTGAAAAGAAAAAGTACAAACTTGATAAGGACTCAGTTATTTTATTAGAACAAATTCGT
ACTTTAGATAAAAATCGTTTAAAAGAAAAACTCACATATTTGTCTGATAACAAAATGAAAGAAGTAGATTACGCCCTAGA
CATTAGTCTAGGTTTACATAATTTCAATCATTCTAAGACATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223578 WP_001307405.1 NZ_CP086500:c612243-611908 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223578 NZ_CP118973:811199-811369 [Staphylococcus warneri]
ATGTTATCTTTTAATCAAAATAGAAGTTACAGTTTAGAGCAATCTTTAAAAGAAGGTTATGCACAAATGGCTGATTTAAA
TCTCTCCCTAGCAACCGAAGCTTTTCCTATTGAATGTGAAGCGTGCGATTGCAATGAAACATATTTAACTTCTAACGCTA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References