Detailed information of TA system TA223574

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25486..25755
• Replicon: plasmid pEc-050-T5-ESBL_4
• Accession: NZ_CP086494
• Organism: Escherichia coli strain Ec-050-T5-ESBL

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LNN51_RS25285
• Protein ID: WP_001372321.1
• Coordinates: 25630..25755 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25486..25551 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN51_RS25245	20767..20894	-	128	Protein_31	hypothetical protein	-
LNN51_RS25250	21196..21723	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LNN51_RS25255	21781..22014	+	234	WP_000006003.1	DUF905 family protein	-
LNN51_RS25260	22075..24098	+	2024	Protein_34	ParB/RepB/Spo0J family partition protein	-
LNN51_RS25265	24167..24601	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LNN51_RS25270	24598..25360	+	763	Protein_36	plasmid SOS inhibition protein A	-
-	25329..25553	+	225	NuclAT_0	-	-
-	25329..25553	+	225	NuclAT_0	-	-
-	25329..25553	+	225	NuclAT_0	-	-
-	25329..25553	+	225	NuclAT_0	-	-
LNN51_RS25275	25338..25517	-	180	WP_001309233.1	hypothetical protein	-
-	25486..25551	-	66	-	-	Antitoxin
LNN51_RS25280	25539..25688	+	150	Protein_38	plasmid maintenance protein Mok	-
LNN51_RS25285	25630..25755	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LNN51_RS25290	26074..26370	-	297	Protein_40	hypothetical protein	-
LNN51_RS25295	26670..26966	+	297	WP_001272251.1	hypothetical protein	-
LNN51_RS25300	27077..27898	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LNN51_RS25305	28195..28797	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
LNN51_RS25310	29120..29503	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LNN51_RS25315	29697..30368	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
LNN51_RS25320	30505..30732	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T223574 WP_001372321.1 NZ_CP086494:25630-25755 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T223574 NZ_CP118964:204432-204788 [Acinetobacter lwoffii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAATCTACTCAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGGCCGAGTTTAGGCCGCCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGCGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT223574 NZ_CP086494:c25551-25486 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin