Detailed information of TA system TA223572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 48375..49086
• Replicon: plasmid pEc-050-T5-ESBL_3
• Accession: NZ_CP086493
• Organism: Escherichia coli strain Ec-050-T5-ESBL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: LNN51_RS24780
• Protein ID: WP_000162415.1
• Coordinates: 48375..48677 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN51_RS24785
• Protein ID: WP_000806445.1
• Coordinates: 48748..49086 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN51_RS24750 (LNN51_24755)	43394..44044	-	651	WP_032153356.1	hypothetical protein	-
LNN51_RS24755 (LNN51_24760)	44058..44963	-	906	WP_001258014.1	recombination-associated protein RdgC	-
LNN51_RS24760 (LNN51_24765)	45023..45676	-	654	WP_000828873.1	hypothetical protein	-
LNN51_RS24765 (LNN51_24770)	45673..46653	-	981	WP_000046499.1	hypothetical protein	-
LNN51_RS24770 (LNN51_24775)	46657..47424	-	768	WP_000203290.1	hypothetical protein	-
LNN51_RS24775 (LNN51_24780)	47421..48212	-	792	WP_000532305.1	hypothetical protein	-
LNN51_RS24780 (LNN51_24785)	48375..48677	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN51_RS24785 (LNN51_24790)	48748..49086	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
LNN51_RS24790 (LNN51_24795)	49143..49328	+	186	Protein_55	hypothetical protein	-
LNN51_RS24795 (LNN51_24800)	49365..50093	+	729	WP_000986754.1	hypothetical protein	-
LNN51_RS24800 (LNN51_24805)	50127..51320	-	1194	WP_102218742.1	phage tail protein	-
LNN51_RS24805 (LNN51_24810)	51313..51642	-	330	WP_000542383.1	hypothetical protein	-
LNN51_RS24810 (LNN51_24815)	51971..52624	-	654	WP_000410952.1	hypothetical protein	-
LNN51_RS24815 (LNN51_24820)	52970..53791	+	822	WP_001427910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89996	89996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T223572 WP_000162415.1 NZ_CP086493:48375-48677 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T223572 NZ_CP118964:c153494-153198 [Acinetobacter lwoffii]
ATGATTGAAATTAAACGTCTGCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATCCGCCTGAA
TCGTCGATTAGATAAGGTTCAACGTGGAAACTGGGGAGACATTAAACCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTTCTGGATGGAGGATGTATTACATCCAACATGGTGACGTTGTGATTGTAATGCTTGGTGGTGGAGATAAATCA
ACTCAACAACAAGACATCGATCGTGCAGTCAAACTATCGAAAACGTTGGAGGATTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT223572 WP_000806445.1 NZ_CP086493:48748-49086 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT223572 NZ_CP118964:c153196-152906 [Acinetobacter lwoffii]
ATGGTTAAAGTAGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATTGTGATTTATCTCAA
TATGGTTCTTGAAGAGAATGATCCAGCTGAATTAGCTCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGGATTGGGCGTGAGGCACTCTACAAAGCCCTACGTCATGATTCAGCACCACGTTTTGATACAATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAACTCACAGTACAACACGCCTAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3