Detailed information of TA system TA022356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 511547..512202
• Replicon: chromosome
• Accession: NC_009727
• Organism: Coxiella burnetii Dugway 5J108-111

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CBUD_RS02705
• Protein ID: WP_011996617.1
• Coordinates: 511864..512202 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CBUD_RS02700
• Protein ID: WP_010958263.1
• Coordinates: 511547..511858 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBUD_RS02675	506822..507499	-	678	WP_011996613.1	cyclin protein	-
CBUD_RS02685	507819..509201	-	1383	WP_010958260.1	cysteine--tRNA ligase	-
CBUD_RS02690	509192..510589	-	1398	WP_011996615.1	glutamate--tRNA ligase	-
CBUD_RS02695	510746..511477	+	732	WP_011996616.1	UDP-2,3-diacylglucosamine diphosphatase	-
CBUD_RS02700	511547..511858	-	312	WP_010958263.1	HigA family addiction module antidote protein	Antitoxin
CBUD_RS02705	511864..512202	-	339	WP_011996617.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBUD_RS02710	512561..513705	+	1145	Protein_529	ISAs1-like element ISCbu1 family transposase	-
CBUD_RS02715	513886..514878	+	993	Protein_530	IS30 family transposase	-
CBUD_RS02720	514967..516337	+	1371	WP_043880840.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (20-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13428.63 Da
• Isoelectric Point: 10.2262

>T22356 WP_011996617.1 NC_009727:c512202-511864 [Coxiella burnetii Dugway 5J108-111]
MPLTLYIMLDVTRKTVILEVMIKSFKDKYTKYLYEGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T22356 NC_009727:c512202-511864 [Coxiella burnetii Dugway 5J108-111]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATGATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATGAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT22356 WP_010958263.1 NC_009727:c511858-511547 [Coxiella burnetii Dugway 5J108-111]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT22356 NC_009727:c511858-511547 [Coxiella burnetii Dugway 5J108-111]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG