Detailed information of TA system TA223556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3786228..3786922
• Replicon: chromosome
• Accession: NZ_CP086490
• Organism: Escherichia coli strain Ec-050-T5-ESBL

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LNN51_RS18220
• Protein ID: WP_001263489.1
• Coordinates: 3786228..3786626 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LNN51_RS18225
• Protein ID: WP_000554758.1
• Coordinates: 3786629..3786922 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3781816)	3781816..3781896	-	81	NuclAT_12	-	-
- (3781816)	3781816..3781896	-	81	NuclAT_12	-	-
- (3781816)	3781816..3781896	-	81	NuclAT_12	-	-
- (3781816)	3781816..3781896	-	81	NuclAT_12	-	-
LNN51_RS18195 (3782492)	3782492..3782950	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LNN51_RS18200 (3783211)	3783211..3784668	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LNN51_RS18205 (3784725)	3784725..3785246	-	522	Protein_3586	peptide chain release factor H	-
LNN51_RS18210 (3785242)	3785242..3785448	-	207	Protein_3587	RtcB family protein	-
LNN51_RS18215 (3785766)	3785766..3786218	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LNN51_RS18220 (3786228)	3786228..3786626	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LNN51_RS18225 (3786629)	3786629..3786922	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LNN51_RS18230 (3786974)	3786974..3788029	-	1056	WP_001226164.1	DNA polymerase IV	-
LNN51_RS18235 (3788100)	3788100..3788885	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LNN51_RS18240 (3788857)	3788857..3790569	+	1713	Protein_3593	flagellar biosynthesis protein FlhA	-
LNN51_RS18245 (3790793)	3790793..3791290	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T223556 WP_001263489.1 NZ_CP086490:c3786626-3786228 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T223556 NZ_CP118962:412934-413242 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT223556 WP_000554758.1 NZ_CP086490:c3786922-3786629 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT223556 NZ_CP118962:412660-412932 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3