Detailed information of TA system TA223524

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59148..59402
• Replicon: plasmid pEc-050-T20-MAC_2
• Accession: NZ_CP086482
• Organism: Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LNN46_RS24300
• Protein ID: WP_001312851.1
• Coordinates: 59148..59297 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	59341..59402 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN46_RS24270 (54395)	54395..55306	-	912	WP_000440183.1	carbamate kinase	-
LNN46_RS24275 (55317)	55317..56537	-	1221	WP_000410951.1	arginine deiminase	-
LNN46_RS24280 (57244)	57244..57858	+	615	Protein_67	VENN motif pre-toxin domain-containing protein	-
LNN46_RS24285 (57858)	57858..58304	-	447	Protein_68	plasmid replication initiator RepA	-
LNN46_RS24290 (58297)	58297..58371	-	75	WP_032336874.1	RepA leader peptide Tap	-
LNN46_RS24295 (58607)	58607..58864	-	258	WP_000083833.1	replication regulatory protein RepA	-
LNN46_RS24300 (59148)	59148..59297	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (59341)	59341..59402	+	62	NuclAT_0	-	Antitoxin
- (59341)	59341..59402	+	62	NuclAT_0	-	Antitoxin
- (59341)	59341..59402	+	62	NuclAT_0	-	Antitoxin
- (59341)	59341..59402	+	62	NuclAT_0	-	Antitoxin
LNN46_RS24305 (59541)	59541..59723	-	183	WP_000968309.1	hypothetical protein	-
LNN46_RS24310 (59824)	59824..60440	+	617	Protein_73	IS1-like element IS1A family transposase	-
LNN46_RS24315 (60478)	60478..62049	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
LNN46_RS24320 (62069)	62069..62416	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
LNN46_RS24325 (62416)	62416..63093	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
LNN46_RS24330 (63148)	63148..63237	+	90	Protein_77	IS1 family transposase	-
LNN46_RS24335 (63538)	63538..63750	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / aac(3)-IIa / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..99476	99476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T223524 WP_001312851.1 NZ_CP086482:c59297-59148 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T223524 NZ_CP118936:1752190-1752522 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT223524 NZ_CP086482:59341-59402 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin