Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3792424..3792965 Replicon chromosome
Accession NZ_CP086480
Organism Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LNN46_RS18270 Protein ID WP_000615983.1
Coordinates 3792687..3792965 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LNN46_RS18265 Protein ID WP_000729703.1
Coordinates 3792424..3792684 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN46_RS18245 3788099..3788884 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LNN46_RS18250 3788856..3790568 + 1713 Protein_3595 flagellar biosynthesis protein FlhA -
LNN46_RS18255 3790792..3791289 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LNN46_RS18260 3791465..3792214 - 750 WP_000056849.1 C40 family peptidase -
LNN46_RS18265 3792424..3792684 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LNN46_RS18270 3792687..3792965 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LNN46_RS18275 3793121..3793861 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LNN46_RS18280 3793832..3794599 - 768 WP_000333380.1 class II glutamine amidotransferase -
LNN46_RS18285 3794805..3795383 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T223515 WP_000615983.1 NZ_CP086480:3792687-3792965 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T223515 NZ_CP118932:c3782699-3782310 [Serratia marcescens]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTTATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCGTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCGGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAAACATGGAGAGAAAGGGCAAAATCATGGGCGCGATGGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGTGC
GACGCTGGTGACCAACGATCGCGCATTCGCTATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT223515 WP_000729703.1 NZ_CP086480:3792424-3792684 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT223515 NZ_CP118932:c3782966-3782703 [Serratia marcescens]
ATGGAAAGGATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAGTTTCTTGGCGCGGAAGATCGTCAGCAAGGGGTTGCAGACCGC
GATCCCTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References