Detailed information of TA system TA223514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3786227..3786921
• Replicon: chromosome
• Accession: NZ_CP086480
• Organism: Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: LNN46_RS18230
• Protein ID: WP_001263489.1
• Coordinates: 3786227..3786625 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LNN46_RS18235
• Protein ID: WP_000554758.1
• Coordinates: 3786628..3786921 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3781815)	3781815..3781895	-	81	NuclAT_12	-	-
- (3781815)	3781815..3781895	-	81	NuclAT_12	-	-
- (3781815)	3781815..3781895	-	81	NuclAT_12	-	-
- (3781815)	3781815..3781895	-	81	NuclAT_12	-	-
LNN46_RS18205 (3782491)	3782491..3782949	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LNN46_RS18210 (3783210)	3783210..3784667	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
LNN46_RS18215 (3784724)	3784724..3785245	-	522	Protein_3588	peptide chain release factor H	-
LNN46_RS18220 (3785241)	3785241..3785447	-	207	Protein_3589	RtcB family protein	-
LNN46_RS18225 (3785765)	3785765..3786217	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
LNN46_RS18230 (3786227)	3786227..3786625	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LNN46_RS18235 (3786628)	3786628..3786921	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LNN46_RS18240 (3786973)	3786973..3788028	-	1056	WP_001226164.1	DNA polymerase IV	-
LNN46_RS18245 (3788099)	3788099..3788884	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
LNN46_RS18250 (3788856)	3788856..3790568	+	1713	Protein_3595	flagellar biosynthesis protein FlhA	-
LNN46_RS18255 (3790792)	3790792..3791289	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T223514 WP_001263489.1 NZ_CP086480:c3786625-3786227 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T223514 NZ_CP118932:c3352159-3351806 [Serratia marcescens]
GTGTGGGAAATTAAAACAACGGATGCATTTGATCATTGGTTCAGATCACTCAGCGATGCCGACCGGGCCTGCGTGCTTGC
TGCATTGATGGTCCTGCGGGAAAAAGGGCCCGGCTTGCCCAGGCCCTATGCCGATACGGTTAAAGGCTCGCGTTACAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGTGGCGAACCGTTGCGGGCATTTTTCGCCTTCGATCCGTACCGCGTCGGCATG
GTGCTTTGCGCGGGCAATAAAGCGGGCGATGAAAAACGTTTTTACGATCGAATGCTGCAGATTGCCGATCGGGAATTCAC
GAATTGGCTGAACACACTAAAAGAGAAGGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT223514 WP_000554758.1 NZ_CP086480:c3786921-3786628 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT223514 NZ_CP118932:c3351801-3351499 [Serratia marcescens]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGAGGAAAAGCCCGAGGTCGTGGCCGAAGCGCAGGCAATGGCGACGGACAT
TATGCTGAACATCCATCTTGCCGAGCTGCGCGAGAAGGTGCAGAAAACCCAGGTTGAAATGGCGCAGGCGCTGGGTATCA
GGCAACCGACCGTCGCCGTGATGGAAAAACCGGGCCGCGATCTGAAACTGTCTTCGCTCAAACGCTATGTTGAGGCGGCG
GGCGGCAAACTGCGCCTGGATGTCGAACTGCCGGACGGTTCGCACTACGAATTTGTGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3