Detailed information of TA system TA223490

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25490..25759
• Replicon: plasmid pEc-050-T20-ESBL_4
• Accession: NZ_CP086474
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LNN47_RS25275
• Protein ID: WP_001372321.1
• Coordinates: 25634..25759 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25490..25555 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS25235	20771..20898	-	128	Protein_29	hypothetical protein	-
LNN47_RS25240	21200..21727	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LNN47_RS25245	21785..22018	+	234	WP_000006003.1	DUF905 family protein	-
LNN47_RS25250	22079..24102	+	2024	Protein_32	ParB/RepB/Spo0J family partition protein	-
LNN47_RS25255	24171..24605	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LNN47_RS25260	24602..25364	+	763	Protein_34	plasmid SOS inhibition protein A	-
-	25333..25557	+	225	NuclAT_0	-	-
-	25333..25557	+	225	NuclAT_0	-	-
-	25333..25557	+	225	NuclAT_0	-	-
-	25333..25557	+	225	NuclAT_0	-	-
LNN47_RS25265	25342..25521	-	180	WP_001309233.1	hypothetical protein	-
-	25490..25555	-	66	-	-	Antitoxin
LNN47_RS25270	25543..25692	+	150	Protein_36	plasmid maintenance protein Mok	-
LNN47_RS25275	25634..25759	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LNN47_RS25280	26078..26374	-	297	Protein_38	hypothetical protein	-
LNN47_RS25285	26674..26970	+	297	WP_001272251.1	hypothetical protein	-
LNN47_RS25290	27081..27902	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LNN47_RS25295	28199..28801	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
LNN47_RS25300	29124..29507	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LNN47_RS25305	29701..30372	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
LNN47_RS25310	30509..30736	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T223490 WP_001372321.1 NZ_CP086474:25634-25759 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T223490 NZ_CP118910:c5413852-5413406 [Mycobacterium marinum]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TTGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGATCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCAGCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGTCGGCTGCCGGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT223490 NZ_CP086474:c25555-25490 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin