Detailed information of TA system TA223488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 7964..8675
• Replicon: plasmid pEc-050-T20-ESBL_3
• Accession: NZ_CP086473
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: LNN47_RS24585
• Protein ID: WP_000162415.1
• Coordinates: 8373..8675 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LNN47_RS24580
• Protein ID: WP_000806445.1
• Coordinates: 7964..8302 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS24550 (LNN47_24555)	3259..4080	-	822	WP_001427910.1	hypothetical protein	-
LNN47_RS24555 (LNN47_24560)	4426..5079	+	654	WP_000410952.1	hypothetical protein	-
LNN47_RS24560 (LNN47_24565)	5408..5737	+	330	WP_000542383.1	hypothetical protein	-
LNN47_RS24565 (LNN47_24570)	5730..6923	+	1194	WP_102218742.1	phage tail protein	-
LNN47_RS24570 (LNN47_24575)	6957..7685	-	729	WP_000986754.1	hypothetical protein	-
LNN47_RS24575 (LNN47_24580)	7722..7907	-	186	Protein_12	hypothetical protein	-
LNN47_RS24580 (LNN47_24585)	7964..8302	-	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
LNN47_RS24585 (LNN47_24590)	8373..8675	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNN47_RS24590 (LNN47_24595)	8838..9629	+	792	WP_000532305.1	hypothetical protein	-
LNN47_RS24595 (LNN47_24600)	9626..10393	+	768	WP_000203290.1	hypothetical protein	-
LNN47_RS24600 (LNN47_24605)	10397..11377	+	981	WP_000046499.1	hypothetical protein	-
LNN47_RS24605 (LNN47_24610)	11374..12027	+	654	WP_000828873.1	hypothetical protein	-
LNN47_RS24610 (LNN47_24615)	12087..12992	+	906	WP_001258014.1	recombination-associated protein RdgC	-
LNN47_RS24615 (LNN47_24620)	13006..13656	+	651	WP_032153356.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89996	89996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T223488 WP_000162415.1 NZ_CP086473:c8675-8373 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T223488 NZ_CP118907:c165978-165667 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT223488 WP_000806445.1 NZ_CP086473:c8302-7964 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT223488 NZ_CP118907:c165670-165251 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3