Detailed information of TA system TA223478

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67924..68188
• Replicon: plasmid pEc-050-T20-ESBL_1
• Accession: NZ_CP086471
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: LNN47_RS23670
• Protein ID: WP_001303307.1
• Coordinates: 68036..68188 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	67924..67986 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS23655 (63163)	63163..65454	-	2292	WP_001289275.1	F-type conjugative transfer protein TrbC	-
LNN47_RS23660 (65447)	65447..66517	-	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
LNN47_RS23665 (66536)	66536..67744	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (67924)	67924..67986	-	63	NuclAT_0	-	Antitoxin
- (67924)	67924..67986	-	63	NuclAT_0	-	Antitoxin
- (67924)	67924..67986	-	63	NuclAT_0	-	Antitoxin
- (67924)	67924..67986	-	63	NuclAT_0	-	Antitoxin
LNN47_RS23670 (68036)	68036..68188	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
LNN47_RS23675 (68260)	68260..68511	-	252	WP_001291965.1	hypothetical protein	-
LNN47_RS26385 (69011)	69011..69106	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
LNN47_RS23685 (69171)	69171..69308	-	138	WP_047088497.1	hypothetical protein	-
LNN47_RS23690 (69353)	69353..70579	-	1227	WP_024186316.1	RNA-guided endonuclease TnpB family protein	-
LNN47_RS23695 (70638)	70638..71042	+	405	WP_001175009.1	IS200/IS605 family transposase	-
LNN47_RS23700 (71374)	71374..71583	-	210	WP_032178830.1	hemolysin expression modulator Hha	-
LNN47_RS23705 (71681)	71681..72343	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / floR / blaTEM-30 / sul1 / qacE / ant(3'')-Ia / dfrA1	-	1..115932	115932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T223478 WP_001303307.1 NZ_CP086471:68036-68188 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T223478 NZ_CP118906:732020-732505 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT223478 NZ_CP086471:c67986-67924 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin