Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3792445..3792986 Replicon chromosome
Accession NZ_CP086470
Organism Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag LNN47_RS18265 Protein ID WP_000615983.1
Coordinates 3792708..3792986 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag LNN47_RS18260 Protein ID WP_000729703.1
Coordinates 3792445..3792705 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN47_RS18240 (3788120) 3788120..3788905 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LNN47_RS18245 (3788877) 3788877..3790589 + 1713 Protein_3594 flagellar biosynthesis protein FlhA -
LNN47_RS18250 (3790813) 3790813..3791310 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LNN47_RS18255 (3791486) 3791486..3792235 - 750 WP_000056849.1 C40 family peptidase -
LNN47_RS18260 (3792445) 3792445..3792705 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LNN47_RS18265 (3792708) 3792708..3792986 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LNN47_RS18270 (3793142) 3793142..3793882 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LNN47_RS18275 (3793853) 3793853..3794620 - 768 WP_000333380.1 class II glutamine amidotransferase -
LNN47_RS18280 (3794826) 3794826..3795404 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T223473 WP_000615983.1 NZ_CP086470:3792708-3792986 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T223473 NZ_CP118902:11428-11853 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT223473 WP_000729703.1 NZ_CP086470:3792445-3792705 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT223473 NZ_CP118902:11201-11431 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References