Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3786248..3786942 Replicon chromosome
Accession NZ_CP086470
Organism Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LNN47_RS18225 Protein ID WP_001263489.1
Coordinates 3786248..3786646 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LNN47_RS18230 Protein ID WP_000554758.1
Coordinates 3786649..3786942 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3781836) 3781836..3781916 - 81 NuclAT_12 - -
- (3781836) 3781836..3781916 - 81 NuclAT_12 - -
- (3781836) 3781836..3781916 - 81 NuclAT_12 - -
- (3781836) 3781836..3781916 - 81 NuclAT_12 - -
LNN47_RS18200 (3782512) 3782512..3782970 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LNN47_RS18205 (3783231) 3783231..3784688 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LNN47_RS18210 (3784745) 3784745..3785266 - 522 Protein_3587 peptide chain release factor H -
LNN47_RS18215 (3785262) 3785262..3785468 - 207 Protein_3588 RtcB family protein -
LNN47_RS18220 (3785786) 3785786..3786238 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
LNN47_RS18225 (3786248) 3786248..3786646 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LNN47_RS18230 (3786649) 3786649..3786942 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LNN47_RS18235 (3786994) 3786994..3788049 - 1056 WP_001226164.1 DNA polymerase IV -
LNN47_RS18240 (3788120) 3788120..3788905 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LNN47_RS18245 (3788877) 3788877..3790589 + 1713 Protein_3594 flagellar biosynthesis protein FlhA -
LNN47_RS18250 (3790813) 3790813..3791310 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T223472 WP_001263489.1 NZ_CP086470:c3786646-3786248 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T223472 NZ_CP118901:c5267425-5267042 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT223472 WP_000554758.1 NZ_CP086470:c3786942-3786649 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT223472 NZ_CP118901:c5267667-5267425 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References