Detailed information of TA system TA223455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 721531..722224
• Replicon: chromosome
• Accession: NZ_CP086470
• Organism: Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LNN47_RS03525
• Protein ID: WP_000415584.1
• Coordinates: 721531..721827 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LNN47_RS03530
• Protein ID: WP_000650107.1
• Coordinates: 721829..722224 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN47_RS03490 (716619)	716619..716933	-	315	WP_000958598.1	putative quinol monooxygenase	-
LNN47_RS03495 (716964)	716964..717545	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LNN47_RS03500 (717864)	717864..718196	+	333	WP_000917685.1	DUF2645 family protein	-
LNN47_RS03505 (718242)	718242..719591	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
LNN47_RS03510 (719588)	719588..720247	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
LNN47_RS03515 (720399)	720399..720791	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LNN47_RS03520 (720844)	720844..721326	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LNN47_RS03525 (721531)	721531..721827	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LNN47_RS03530 (721829)	721829..722224	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LNN47_RS03535 (722357)	722357..723964	+	1608	WP_087523553.1	ABC transporter substrate-binding protein	-
LNN47_RS03540 (724102)	724102..726360	+	2259	WP_032329482.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T223455 WP_000415584.1 NZ_CP086470:721531-721827 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T223455 NZ_CP118865:c82389-82078 [Providencia rettgeri]
ATGCACGTAATATCAAGAGAACCTTTTGACCAGGCATCCAAGCGATTTCCAAACAGTGCTCAGGCGCTATCTGACATATA
TCGAACACTCAAGCGCGACAGTTATCAAACGCCTGATGAGCTTAAAAAGGTTTTCCCCAGCCTTGATCGGATGAAGTATA
GAGAGAAATGGTGGGTCATCGACATCAGTGGCAATTCATTAAGAATGATGTTTTTTGCCGACTTTGACCGGGGGAAAATA
TTCGTGAAACATATAACAACCCACGCTGAATACGATAGGTTGACTGATCACTACCGGAGAACAAAAGCATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT223455 WP_000650107.1 NZ_CP086470:721829-722224 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT223455 NZ_CP118865:c82081-81665 [Providencia rettgeri]
ATGATGTTTCAAGATGCAGTAAACGCAGCACAAAACCTGATAAGCATTGTGCCGTTATTGGGAAACAGCCATTCTCGCGA
AGACTATGAAAAAGCCGTTCAACTGGTTGAGCACCTTGTCGAAAATGACCCAGACAACCCGCTTATCGACTTGATTTGCG
CGAAGATTGATGCGTATGAAAAAACAGCGCCCGAATTTGCTGAATTTAACGAGCGCTTAGCAAAAAGCAATGGCGGTGTA
GCAGCATTGAGAACGCTGATGGATCAATACCACCTTAATACGACAGACTTTCAAGATGAGCTCGGTTCGCGCTCATACGT
TAGCCGCATCCTCAACGGTGAACGAGGATTAACACTGGAGCACATTAAAAAACTGTCTGCGCGTTTTAATATCCCTGCCT
CCATTTTCATAGACTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65