Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 655567..656294 Replicon chromosome
Accession NZ_CP086470
Organism Escherichia coli strain Ec-050-T20-ESBL

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LNN47_RS03205 Protein ID WP_000550189.1
Coordinates 655567..655881 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LNN47_RS03210 Protein ID WP_000560266.1
Coordinates 655878..656294 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN47_RS03185 (651725) 651725..652711 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
LNN47_RS03190 (652790) 652790..653482 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
LNN47_RS03195 (653559) 653559..654062 - 504 WP_001333820.1 M48 family metallopeptidase -
LNN47_RS03200 (654147) 654147..655283 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LNN47_RS03205 (655567) 655567..655881 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LNN47_RS03210 (655878) 655878..656294 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LNN47_RS03215 (656339) 656339..658357 - 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LNN47_RS03220 (658783) 658783..661134 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T223454 WP_000550189.1 NZ_CP086470:655567-655881 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T223454 NZ_CP118865:42932-43444 [Providencia rettgeri]
ATGACATTGAAGCTGACAGCACCCGAACCCCTCAAAACAGGCCATTTGCTGGAAGGCTTTTCATCTGGTGAAGAGGCTTT
AGATCTCTGGCTAAAAAATCGGGCCATGAGTAATCAAAACTCAGGAGCTTCACGAACATTTGTAACCACTTCTGATAACC
AAGTGATGGGGTATTATGCCTTGTCTTCCGGGATTGTGAGCACCAATCAAGCTGTTGGCCGATTCCGCCGCAATATGCCT
TCTGACATACCAGTCATCCTTCTCGGTAGACTGGCAGTGGATACAAGAGCTAAAGGGCTTGGAGTTGGAAGGGGATTGGT
CAAAGATGCCTGTCATCGTGTTATTCAGGCCTCTGGGCTGGTGGGCATACGAGGAATAGTGGTCCACGCTCTAACTGATG
ATGCTAAACGCTTCTATGAACACATTGGTTTCGTCCCTTCACCACTTGACCCTATGATGCTAATGATAACACTCGCCGAT
CTGCAACTCGCAATGGGTATTCATCCAAACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT223454 WP_000560266.1 NZ_CP086470:655878-656294 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT223454 NZ_CP118865:42648-42935 [Providencia rettgeri]
ATGGCAACTCAAAATGATGTAGCGGTAACACGCTCATCTCTGAACCTGAGAATTAAACCGGAAGACAAAATCCTGATCGA
CCGCGCCGCAAATGCGGTAGGCAAAAATCGAACTGAGTTCGTTCTGGAAGCCGCTCGACGAGCTGCTGAAGAGACACTGG
CGGACCTGCGGGTTATTAACGTCAGCCCCGAGGTATACCAGGAGTTTATTAATCAGCTTGATGCCGCACCAAGGAACAAT
GAGGCATTACGAAAAACATTGATGTCTAAATCCCCTTGGGAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References