Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2554757..2555446 Replicon chromosome
Accession NZ_CP086461
Organism Klebsiella pneumoniae strain MCL-2017-2-T5-ESBL

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag LNN56_RS12465 Protein ID WP_021469727.1
Coordinates 2555129..2555446 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag LNN56_RS12460 Protein ID WP_020804705.1
Coordinates 2554757..2555053 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN56_RS12445 (2551149) 2551149..2552531 + 1383 WP_004151222.1 MFS transporter -
LNN56_RS12450 (2552575) 2552575..2554191 + 1617 WP_004175961.1 carbohydrate porin -
LNN56_RS12455 (2554232) 2554232..2554678 - 447 WP_032435212.1 hypothetical protein -
LNN56_RS12460 (2554757) 2554757..2555053 - 297 WP_020804705.1 NadS family protein Antitoxin
LNN56_RS12465 (2555129) 2555129..2555446 - 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LNN56_RS12470 (2555653) 2555653..2555880 - 228 WP_002906690.1 tautomerase PptA -
LNN56_RS12475 (2555951) 2555951..2556568 - 618 WP_032425015.1 glutathione S-transferase family protein -
LNN56_RS12480 (2556646) 2556646..2557497 - 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
LNN56_RS12485 (2557536) 2557536..2558315 - 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
LNN56_RS12490 (2558299) 2558299..2559225 - 927 WP_032435210.1 amino acid ABC transporter permease -
LNN56_RS12495 (2559235) 2559235..2559744 - 510 WP_020802144.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T223397 WP_021469727.1 NZ_CP086461:c2555446-2555129 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T223397 NZ_CP118830:c2123660-2123298 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT223397 WP_020804705.1 NZ_CP086461:c2555053-2554757 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT223397 NZ_CP118830:c2123827-2123657 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References