Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2554758..2555447 Replicon chromosome
Accession NZ_CP086455
Organism Klebsiella pneumoniae strain MCL-2017-2-T20-ESBL

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag LNN48_RS12470 Protein ID WP_021469727.1
Coordinates 2555130..2555447 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag LNN48_RS12465 Protein ID WP_020804705.1
Coordinates 2554758..2555054 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN48_RS12450 (2551150) 2551150..2552532 + 1383 WP_004151222.1 MFS transporter -
LNN48_RS12455 (2552576) 2552576..2554192 + 1617 WP_004175961.1 carbohydrate porin -
LNN48_RS12460 (2554233) 2554233..2554679 - 447 WP_032435212.1 hypothetical protein -
LNN48_RS12465 (2554758) 2554758..2555054 - 297 WP_020804705.1 NadS family protein Antitoxin
LNN48_RS12470 (2555130) 2555130..2555447 - 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LNN48_RS12475 (2555654) 2555654..2555881 - 228 WP_002906690.1 tautomerase PptA -
LNN48_RS12480 (2555952) 2555952..2556569 - 618 WP_032425015.1 glutathione S-transferase family protein -
LNN48_RS12485 (2556647) 2556647..2557498 - 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
LNN48_RS12490 (2557537) 2557537..2558316 - 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
LNN48_RS12495 (2558300) 2558300..2559226 - 927 WP_032435210.1 amino acid ABC transporter permease -
LNN48_RS12500 (2559236) 2559236..2559745 - 510 WP_020802144.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T223357 WP_021469727.1 NZ_CP086455:c2555447-2555130 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T223357 NZ_CP118755:2762215-2762358 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAACCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT223357 WP_020804705.1 NZ_CP086455:c2555054-2554758 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT223357 NZ_CP118755:c2762104-2762041 [Enterococcus faecalis]
TTGTGCTATAATAGCAATGAAAAGAGAGATATGCTGTAACATACCTCTCTGATGTAGAGCCGTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References