Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 334457..335043 Replicon chromosome
Accession NZ_CP086455
Organism Klebsiella pneumoniae strain MCL-2017-2-T20-ESBL

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LNN48_RS01590 Protein ID WP_002920800.1
Coordinates 334675..335043 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LNN48_RS01585 Protein ID WP_004174006.1
Coordinates 334457..334678 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN48_RS01565 (330614) 330614..331540 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LNN48_RS01570 (331537) 331537..332814 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LNN48_RS01575 (332811) 332811..333578 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LNN48_RS01580 (333580) 333580..334293 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LNN48_RS01585 (334457) 334457..334678 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LNN48_RS01590 (334675) 334675..335043 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LNN48_RS01595 (335316) 335316..336632 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LNN48_RS01600 (336739) 336739..337626 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LNN48_RS01605 (337623) 337623..338468 + 846 WP_032434559.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LNN48_RS01610 (338470) 338470..339540 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 331537..340277 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T223353 WP_002920800.1 NZ_CP086455:334675-335043 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T223353 NZ_CP118755:550377-550493 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT223353 WP_004174006.1 NZ_CP086455:334457-334678 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT223353 NZ_CP118755:c550609-550404 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCGTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References