Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4013096..4013693 Replicon chromosome
Accession NZ_CP086439
Organism Klebsiella pneumoniae strain AE-2247421-T5-MAC

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LNN53_RS19710 Protein ID WP_004142563.1
Coordinates 4013376..4013693 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LNN53_RS19705 Protein ID WP_004142561.1
Coordinates 4013096..4013383 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN53_RS19675 (4009177) 4009177..4009425 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LNN53_RS19680 (4009443) 4009443..4009784 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LNN53_RS19685 (4009815) 4009815..4010930 - 1116 Protein_3875 MBL fold metallo-hydrolase -
LNN53_RS19690 (4011109) 4011109..4011690 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
LNN53_RS19695 (4011690) 4011690..4012058 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LNN53_RS19700 (4012178) 4012178..4012831 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
LNN53_RS19705 (4013096) 4013096..4013383 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LNN53_RS19710 (4013376) 4013376..4013693 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LNN53_RS19715 (4013878) 4013878..4014921 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
LNN53_RS19720 (4015588) 4015588..4016454 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
LNN53_RS19725 (4016563) 4016563..4017990 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T223325 WP_004142563.1 NZ_CP086439:c4013693-4013376 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T223325 NZ_CP118687:c437984-437739 [Rhizobium sp. MJ22]
ATGAAAGAGATCGCCTACAGCAAGGCTGCCGCGAAGGCACTCCGCAAGATGCAGCCGAAGCGCGCGGCCGCGATCGTCGC
CAAGGTCGAAGCCTTCGCCAACAGCGAGCCGGTTGATCTCAAGAAACTGCAAGGCAGCGAATTTTTTCGCATTCGCGTCG
GACAGGATCGAGTCATCATCGACGATCAGACCATGCAGGTCCTCGTCATCAAAGCCGGCCCGCGTGGCGACATCTATAAC
GAATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT223325 WP_004142561.1 NZ_CP086439:c4013383-4013096 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT223325 NZ_CP118687:c437729-437382 [Rhizobium sp. MJ22]
ATGGGTGAAATTCAGACACTGACCATCGACGGCAAGGGCTATGTCCTGCTCTCCGAAGAAGACTACTGCGACCTTGTCGA
CCTCAGCGAGGCGCGCGCCGCGAAGGCGCGCATCGATGCCGGCGAAGAGACCTGGCCGGAAGACGTCGTCAAGGCGCTGA
TTTCAGGCGAAGATCCTGTCCGCGTGTTCCGCAAGCATCGCGGCATGAGCGTCAAGGAGCTGGCGGAAAAAGCCGGGCTC
TCACAGCCCTATCTTTCGGAAATCGAGACCGGAAAGAAGGAAGGCTCCCTGGACGCATTGAAGGCGATCGCTCGGATTCT
GGACGTCGACCTCGACGATCTGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References